Opin vísindi

Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland

Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland


Titill: Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland
Höfundur: Einarsdóttir, Eydís ; Magnúsdóttir, Manuela ; Astarita, Giuseppe ; Köck, Matthias ; Ögmundsdóttir, Helga M. ; Þorsteinsdóttir, Margrét ; Rapp, Hans ; Omarsdottir, Sesselja ; Paglia, Giuseppe
Útgáfa: 2017-02-22
Tungumál: Enska
Umfang: 52
Háskóli/Stofnun: Háskóli Íslands
University of Iceland
Svið: Heilbrigðisvísindasvið (HÍ)
School of Health Sciences (UI)
Verkfræði- og náttúruvísindasvið (HÍ)
School of Engineering and Natural Sciences (UI)
Deild: Lyfjafræðideild (HÍ)
Faculty of Pharmaceutical Sciences (UI)
Rannsóknarsetur í kerfislíffræði (HÍ)
Center for Systems Biology (UI)
Læknadeild (HÍ)
Faculty of Medicine (UI)
Birtist í: Marine Drugs;15(3)
ISSN: 1660-3397
DOI: 10.3390/md15020052
Efnisorð: Marine natural products; Sponges; Metabolomics; Ion mobility; Haliclona rosea; Sjávarafurðir; Svampdýr; Efnaskipti
URI: https://hdl.handle.net/20.500.11815/340

Skoða fulla færslu

Tilvitnun:

Einarsdottir E, Magnusdottir M, Astarita G, Köck M, Ögmundsdottir HM, Thorsteinsdottir M, Rapp HT, Omarsdottir S, Paglia G. Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland. Marine Drugs. 2017; 15(2):52. doi:10.3390/md15020052

Útdráttur:

Twenty-eight sponge specimens were collected at a shallow water hydrothermal vent site north of Iceland. Extracts were prepared and tested in vitro for cytotoxic activity, and eight of them were shown to be cytotoxic. A mass spectrometry (MS)-based metabolomics approach was used to determine the chemical composition of the extracts. This analysis highlighted clear differences in the metabolomes of three sponge specimens, and all of them were identified as Haliclona (Rhizoniera) rosea (Bowerbank, 1866). Therefore, these specimens were selected for further investigation. Haliclona rosea metabolomes contained a class of potential key compounds, the 3-alkyl pyridine alkaloids (3-APA) responsible for the cytotoxic activity of the fractions. Several 3-APA compounds were tentatively identified including haliclamines, cyclostellettamines, viscosalines and viscosamines. Among these compounds, cyclostellettamine P was tentatively identified for the first time by using ion mobility MS in time-aligned parallel (TAP) fragmentation mode. In this work, we show the potential of applying metabolomics strategies and in particular the utility of coupling ion mobility with MS for the molecular characterization of sponge specimens.

Leyfi:

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Skrár

Þetta verk birtist í eftirfarandi safni/söfnum:


Fletta

Um vefinn

Reikningurinn minn