Opin vísindi

Component-resolved microarray analysis of IgE sensitization profiles to Culicoides recombinant allergens in horses with insect bite hypersensitivity

Show simple item record

dc.contributor The Institute for Experimental Pathology University of Iceland
dc.contributor University of Iceland
dc.contributor.author Novotny, Ella N.
dc.contributor.author White, Samuel J.
dc.contributor.author Wilson, A. Douglas
dc.contributor.author Stefánsdóttir, Sara Björk
dc.contributor.author Tijhaar, Edwin
dc.contributor.author Jónsdóttir, Sigríður
dc.contributor.author Frey, Rebekka
dc.contributor.author Reiche, Dania
dc.contributor.author Rose, Horst
dc.contributor.author Rhyner, Claudio
dc.contributor.author Schüpbach-Regula, Gertraud
dc.contributor.author Þorsteinsdóttir, Sigurbjörg
dc.contributor.author Alcocer, Marcos
dc.contributor.author Marti, Eliane
dc.date.accessioned 2022-04-30T01:04:03Z
dc.date.available 2022-04-30T01:04:03Z
dc.date.issued 2021-04
dc.identifier.citation Novotny , E N , White , S J , Wilson , A D , Stefánsdóttir , S B , Tijhaar , E , Jónsdóttir , S , Frey , R , Reiche , D , Rose , H , Rhyner , C , Schüpbach-Regula , G , Þorsteinsdóttir , S , Alcocer , M & Marti , E 2021 , ' Component-resolved microarray analysis of IgE sensitization profiles to Culicoides recombinant allergens in horses with insect bite hypersensitivity ' , Allergy: European Journal of Allergy and Clinical Immunology , vol. 76 , no. 4 , pp. 1147-1157 . https://doi.org/10.1111/all.14556
dc.identifier.issn 0105-4538
dc.identifier.other PURE: 44795932
dc.identifier.other PURE UUID: 6be28124-1f21-4b3b-9fc7-1955ee1414b1
dc.identifier.other Scopus: 85090161303
dc.identifier.uri https://hdl.handle.net/20.500.11815/3115
dc.description Publisher Copyright: © 2020 The Authors. Allergy published by European Academy of Allergy and Clinical Immunology and John Wiley & Sons Ltd.
dc.description.abstract Background: Allergy to bites of blood-sucking insects, including biting midges, can affect both human and veterinary patients. Horses are often suffering from an IgE-mediated allergic dermatitis caused by bites of midges (Culicoides spp). With the aim to improve allergen immunotherapy (AIT), numerous Culicoides allergens have been produced as recombinant (r-) proteins. This study aimed to test a comprehensive panel of differently expressed Culicoides r-allergens on a cohort of IBH-affected and control horses using an allergen microarray. Methods: IgE levels to 27 Culicoides r-allergens, including 8 previously unpublished allergens, of which 11 were expressed in more than one expression system, were determined in sera from 347 horses. ROC analyses were carried out, cut-offs selected using a specificity of 95% and seropositivity rates compared between horses affected with insect bite hypersensitivity (IBH) and control horses. The combination of r-allergens giving the best performing test was determined using logistic regression analysis. Results: Seropositivity was significantly higher in IBH horses compared with controls for 25 r-allergens. Nine Culicoides r-allergens were major allergens for IBH with seven of them binding IgE in sera from > 70% of the IBH-affected horses. Combination of these top seven r-allergens could diagnose > 90% of IBH-affected horses with a specificity of > 95%. Correlation between differently expressed r-allergens was usually high (mean = 0.69, range: 0.28-0.91). Conclusion: This microarray will be a powerful tool for the development of component-resolved, patient-tailored AIT for IBH and could be useful for the study of allergy to biting midges in humans and other species.
dc.format.extent 11
dc.format.extent 1147-1157
dc.language.iso en
dc.relation.ispartofseries Allergy: European Journal of Allergy and Clinical Immunology; 76(4)
dc.rights info:eu-repo/semantics/openAccess
dc.subject culicoides allergens
dc.subject equine insect bite hypersensitivity
dc.subject IgE
dc.subject microarray
dc.subject Immunology and Allergy
dc.subject Immunology
dc.title Component-resolved microarray analysis of IgE sensitization profiles to Culicoides recombinant allergens in horses with insect bite hypersensitivity
dc.type /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article
dc.description.version Peer reviewed
dc.identifier.pmid 32780483
dc.identifier.doi https://doi.org/10.1111/all.14556
dc.relation.url http://www.scopus.com/inward/record.url?scp=85090161303&partnerID=8YFLogxK
dc.contributor.department Faculty of Medicine


Files in this item

This item appears in the following Collection(s)

Show simple item record