dc.contributor |
The Institute for Experimental Pathology University of Iceland |
dc.contributor |
University of Iceland |
dc.contributor.author |
Novotny, Ella N. |
dc.contributor.author |
White, Samuel J. |
dc.contributor.author |
Wilson, A. Douglas |
dc.contributor.author |
Stefánsdóttir, Sara Björk |
dc.contributor.author |
Tijhaar, Edwin |
dc.contributor.author |
Jónsdóttir, Sigríður |
dc.contributor.author |
Frey, Rebekka |
dc.contributor.author |
Reiche, Dania |
dc.contributor.author |
Rose, Horst |
dc.contributor.author |
Rhyner, Claudio |
dc.contributor.author |
Schüpbach-Regula, Gertraud |
dc.contributor.author |
Þorsteinsdóttir, Sigurbjörg |
dc.contributor.author |
Alcocer, Marcos |
dc.contributor.author |
Marti, Eliane |
dc.date.accessioned |
2022-04-30T01:04:03Z |
dc.date.available |
2022-04-30T01:04:03Z |
dc.date.issued |
2021-04 |
dc.identifier.citation |
Novotny , E N , White , S J , Wilson , A D , Stefánsdóttir , S B , Tijhaar , E , Jónsdóttir , S , Frey , R , Reiche , D , Rose , H , Rhyner , C , Schüpbach-Regula , G , Þorsteinsdóttir , S , Alcocer , M & Marti , E 2021 , ' Component-resolved microarray analysis of IgE sensitization profiles to Culicoides recombinant allergens in horses with insect bite hypersensitivity ' , Allergy: European Journal of Allergy and Clinical Immunology , vol. 76 , no. 4 , pp. 1147-1157 . https://doi.org/10.1111/all.14556 |
dc.identifier.issn |
0105-4538 |
dc.identifier.other |
44795932 |
dc.identifier.other |
6be28124-1f21-4b3b-9fc7-1955ee1414b1 |
dc.identifier.other |
85090161303 |
dc.identifier.other |
32780483 |
dc.identifier.uri |
https://hdl.handle.net/20.500.11815/3115 |
dc.description |
Publisher Copyright: © 2020 The Authors. Allergy published by European Academy of Allergy and Clinical Immunology and John Wiley & Sons Ltd. |
dc.description.abstract |
Background: Allergy to bites of blood-sucking insects, including biting midges, can affect both human and veterinary patients. Horses are often suffering from an IgE-mediated allergic dermatitis caused by bites of midges (Culicoides spp). With the aim to improve allergen immunotherapy (AIT), numerous Culicoides allergens have been produced as recombinant (r-) proteins. This study aimed to test a comprehensive panel of differently expressed Culicoides r-allergens on a cohort of IBH-affected and control horses using an allergen microarray. Methods: IgE levels to 27 Culicoides r-allergens, including 8 previously unpublished allergens, of which 11 were expressed in more than one expression system, were determined in sera from 347 horses. ROC analyses were carried out, cut-offs selected using a specificity of 95% and seropositivity rates compared between horses affected with insect bite hypersensitivity (IBH) and control horses. The combination of r-allergens giving the best performing test was determined using logistic regression analysis. Results: Seropositivity was significantly higher in IBH horses compared with controls for 25 r-allergens. Nine Culicoides r-allergens were major allergens for IBH with seven of them binding IgE in sera from > 70% of the IBH-affected horses. Combination of these top seven r-allergens could diagnose > 90% of IBH-affected horses with a specificity of > 95%. Correlation between differently expressed r-allergens was usually high (mean = 0.69, range: 0.28-0.91). Conclusion: This microarray will be a powerful tool for the development of component-resolved, patient-tailored AIT for IBH and could be useful for the study of allergy to biting midges in humans and other species. |
dc.format.extent |
11 |
dc.format.extent |
1259302 |
dc.format.extent |
1147-1157 |
dc.language.iso |
en |
dc.relation.ispartofseries |
Allergy: European Journal of Allergy and Clinical Immunology; 76(4) |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.subject |
culicoides allergens |
dc.subject |
equine insect bite hypersensitivity |
dc.subject |
IgE |
dc.subject |
microarray |
dc.subject |
Immunology and Allergy |
dc.subject |
Immunology |
dc.title |
Component-resolved microarray analysis of IgE sensitization profiles to Culicoides recombinant allergens in horses with insect bite hypersensitivity |
dc.type |
/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article |
dc.description.version |
Peer reviewed |
dc.identifier.doi |
10.1111/all.14556 |
dc.relation.url |
http://www.scopus.com/inward/record.url?scp=85090161303&partnerID=8YFLogxK |
dc.contributor.department |
Faculty of Medicine |