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Component-resolved microarray analysis of IgE sensitization profiles to Culicoides recombinant allergens in horses with insect bite hypersensitivity

Component-resolved microarray analysis of IgE sensitization profiles to Culicoides recombinant allergens in horses with insect bite hypersensitivity


Title: Component-resolved microarray analysis of IgE sensitization profiles to Culicoides recombinant allergens in horses with insect bite hypersensitivity
Author: Novotny, Ella N.
White, Samuel J.
Wilson, A. Douglas
Stefánsdóttir, Sara Björk
Tijhaar, Edwin
Jónsdóttir, Sigríður
Frey, Rebekka
Reiche, Dania
Rose, Horst
Rhyner, Claudio
... 4 more authors Show all authors
Date: 2021-04
Language: English
Scope: 11
University/Institute: The Institute for Experimental Pathology University of Iceland
University of Iceland
Department: Faculty of Medicine
Series: Allergy: European Journal of Allergy and Clinical Immunology; 76(4)
ISSN: 0105-4538
DOI: https://doi.org/10.1111/all.14556
Subject: culicoides allergens; equine insect bite hypersensitivity; IgE; microarray; Immunology and Allergy; Immunology
URI: https://hdl.handle.net/20.500.11815/3115

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Citation:

Novotny , E N , White , S J , Wilson , A D , Stefánsdóttir , S B , Tijhaar , E , Jónsdóttir , S , Frey , R , Reiche , D , Rose , H , Rhyner , C , Schüpbach-Regula , G , Þorsteinsdóttir , S , Alcocer , M & Marti , E 2021 , ' Component-resolved microarray analysis of IgE sensitization profiles to Culicoides recombinant allergens in horses with insect bite hypersensitivity ' , Allergy: European Journal of Allergy and Clinical Immunology , vol. 76 , no. 4 , pp. 1147-1157 . https://doi.org/10.1111/all.14556

Abstract:

Background: Allergy to bites of blood-sucking insects, including biting midges, can affect both human and veterinary patients. Horses are often suffering from an IgE-mediated allergic dermatitis caused by bites of midges (Culicoides spp). With the aim to improve allergen immunotherapy (AIT), numerous Culicoides allergens have been produced as recombinant (r-) proteins. This study aimed to test a comprehensive panel of differently expressed Culicoides r-allergens on a cohort of IBH-affected and control horses using an allergen microarray. Methods: IgE levels to 27 Culicoides r-allergens, including 8 previously unpublished allergens, of which 11 were expressed in more than one expression system, were determined in sera from 347 horses. ROC analyses were carried out, cut-offs selected using a specificity of 95% and seropositivity rates compared between horses affected with insect bite hypersensitivity (IBH) and control horses. The combination of r-allergens giving the best performing test was determined using logistic regression analysis. Results: Seropositivity was significantly higher in IBH horses compared with controls for 25 r-allergens. Nine Culicoides r-allergens were major allergens for IBH with seven of them binding IgE in sera from > 70% of the IBH-affected horses. Combination of these top seven r-allergens could diagnose > 90% of IBH-affected horses with a specificity of > 95%. Correlation between differently expressed r-allergens was usually high (mean = 0.69, range: 0.28-0.91). Conclusion: This microarray will be a powerful tool for the development of component-resolved, patient-tailored AIT for IBH and could be useful for the study of allergy to biting midges in humans and other species.

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Publisher Copyright: © 2020 The Authors. Allergy published by European Academy of Allergy and Clinical Immunology and John Wiley & Sons Ltd.

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