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Neonatal Immunization with a Single IL-4/Antigen Dose Induces Increased Antibody Responses after Challenge Infection with Equine Herpesvirus Type 1 (EHV-1) at Weanling Age

Neonatal Immunization with a Single IL-4/Antigen Dose Induces Increased Antibody Responses after Challenge Infection with Equine Herpesvirus Type 1 (EHV-1) at Weanling Age


Titill: Neonatal Immunization with a Single IL-4/Antigen Dose Induces Increased Antibody Responses after Challenge Infection with Equine Herpesvirus Type 1 (EHV-1) at Weanling Age
Höfundur: Wagner, Bettina
Perkins, Gillian
Babasyan, Susanna
Freer, Heather
Keggan, Alison
Goodman, Laura B.
Glaser, Amy
Þorsteinsdóttir, Sigurbjörg
Svansson, Vilhjálmur   orcid.org/0000-0002-3984-4441
Björnsdóttir, Sigríður
Útgáfa: 2017-01-03
Tungumál: Enska
Umfang: e0169072
Háskóli/Stofnun: Háskóli Íslands
University of Iceland
Deild: Tilraunastöð í meinafræði að Keldum (HÍ)
Institute for Experimental Pathology, Keldur (UI)
Birtist í: PLoS ONE;12(1)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0169072
Efnisorð: Antibodies; Neonates; Vaccines; Horses; Mótefni; Nýburar; Bóluefni; Hestar; Frumur
URI: https://hdl.handle.net/20.500.11815/299

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Tilvitnun:

Wagner B, Perkins G, Babasyan S, Freer H, Keggan A, Goodman LB, et al. (2017) Neonatal Immunization with a Single IL-4/Antigen Dose Induces Increased Antibody Responses after Challenge Infection with Equine Herpesvirus Type 1 (EHV-1) at Weanling Age. PLoS ONE 12(1): e0169072. https://doi.org/10.1371/journal.pone.0169072

Útdráttur:

Neonatal foals respond poorly to conventional vaccines. These vaccines typically target T-helper (Th) cell dependent B-cell activation. However, Th2-cell immunity is impaired in foals during the first three months of life. In contrast, neonatal basophils are potent interleukin-4 (IL-4) producers. The purpose of this study was to develop a novel vaccine triggering the natural capacity of neonatal basophils to secrete IL-4 and to evaluate if vaccination resulted in B-cell activation and antibody production against EHV-1 glycoprotein C (gC). Neonatal vaccination was performed by oral biotinylated IgE (IgE-bio) treatment at birth followed by intramuscular injection of a single dose of streptavidin-conjugated gC/IL-4 fusion protein (Sav-gC/IL-4) for crosslinking of receptor-bound IgE-bio (group 1). Neonates in group 2 received the intramuscular Sav-gC/IL-4 vaccine only. Group 3 remained non-vaccinated at birth. After vaccination, gC antibody production was not detectable. The ability of the vaccine to induce protection was evaluated by an EHV-1 challenge infection after weaning at 7 months of age. Groups 1 and 2 responded to EHV-1 infection with an earlier onset and overall significantly increased anti-gC serum antibody responses compared to control group 3. In addition, group 1 weanlings had a decreased initial fever peak after infection indicating partial protection from EHV-1 infection. This suggested that the neonatal vaccination induced a memory B-cell response at birth that was recalled at weanling age after EHV-1 challenge. In conclusion, early stimulation of neonatal immunity via the innate arm of the immune system can induce partial protection and increased antibody responses against EHV-1.

Leyfi:

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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