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Naturally occurring glycosidases in milk from native cattle breeds : Activity and consequences on free and protein bound-glycans

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dc.contributor.author Sunds, Anne Vuholm
dc.contributor.author Roland, Ida Schwartz
dc.contributor.author Sundekilde, Ulrik Kræmer
dc.contributor.author Thesbjerg, Martin Nørmark
dc.contributor.author Robinson, Randall
dc.contributor.author Bunyatratchata, Apichaya
dc.contributor.author Glantz, Maria
dc.contributor.author Paulsson, Marie
dc.contributor.author Leskauskaite, Daiva
dc.contributor.author Pihlanto, Anne
dc.contributor.author Inglingstad, Ragnhild
dc.contributor.author Devold, Tove Gulbrandsen
dc.contributor.author Vegarud, Gerd Elisabeth
dc.contributor.author Birgisdóttir, Bryndís Eva
dc.contributor.author Guðjónsdóttir, María
dc.contributor.author Barile, Daniela
dc.contributor.author Larsen, Lotte Bach
dc.contributor.author Poulsen, Nina Aagaard
dc.date.accessioned 2021-12-15T01:04:19Z
dc.date.available 2021-12-15T01:04:19Z
dc.date.issued 2021-09-28
dc.identifier.citation Sunds , A V , Roland , I S , Sundekilde , U K , Thesbjerg , M N , Robinson , R , Bunyatratchata , A , Glantz , M , Paulsson , M , Leskauskaite , D , Pihlanto , A , Inglingstad , R , Devold , T G , Vegarud , G E , Birgisdóttir , B E , Guðjónsdóttir , M , Barile , D , Larsen , L B & Poulsen , N A 2021 , ' Naturally occurring glycosidases in milk from native cattle breeds : Activity and consequences on free and protein bound-glycans ' , Metabolites , vol. 11 , no. 10 , 662 . https://doi.org/10.3390/metabo11100662
dc.identifier.issn 2218-1989
dc.identifier.other PURE: 40773836
dc.identifier.other PURE UUID: 49d05fda-679c-4cd2-93d6-6e422d69ad8c
dc.identifier.other Scopus: 85116125407
dc.identifier.other unpaywall: 10.3390/metabo11100662
dc.identifier.uri https://hdl.handle.net/20.500.11815/2742
dc.description Funding: This research was funded by the Aarhus University Research Foundation (AUFF, Aarhus, Denmark), Frands Christian Frantsen Fund (Aarhus, Denmark), the Graduate School of Science and Technology (GSST) at Aarhus University (Aarhus, Denmark), as well as the USDA NIFA project C?−DFST-2187-H. Acknowledgments: The Aarhus University Research Foundation (AUFF, Aarhus, Denmark), Frands Christian Frantsen Fund (Aarhus, Denmark), as well as the Graduate School of Science and Technology (GSST) at Aarhus University (Aarhus, Denmark) are acknowledged for the financial support of the study. The authors from University of California, Davis are grateful for the support from the USD? NIF? project C?−DFST-2187-H. Thank you to Gitte Hald Kristiansen for helping out with the LC-MS method. We further thank the farmers and co-workers for collection of milk samples from the native breeds. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
dc.description.abstract Little is known about the extent of variation and activity of naturally occurring milk glycosidases and their potential to degrade milk glycans. A multi-omics approach was used to investigate the relationship between glycosidases and important bioactive compounds such as free oligo-saccharides and O-linked glycans in bovine milk. Using 4-methylumbelliferone (4-MU) assays activities of eight indigenous glycosidases were determined, and by mass spectrometry and1H NMR spectroscopy various substrates and metabolite products were quantified in a subset of milk samples from eight native North European cattle breeds. The results showed a clear variation in glycosidase activities among the native breeds. Interestingly, negative correlations between some glycosidases including β-galactosidase, N-acetyl-β-D-glucosaminidase, certain oligosaccharide isomers as well as O-linked glycans of κ-casein were revealed. Further, a positive correlation was found for free fucose content and α-fucosidase activity (r = 0.37, P-value < 0.001) indicating cleavage of fucosylated glycans in milk at room temperature. The results obtained suggest that milk glycosidases might partially degrade valuable glycans, which would result in lower recovery of glycans and thus represent a loss for the dairy ingredients industry if these activities are pronounced.
dc.format.extent
dc.language.iso en
dc.relation.ispartofseries Metabolites; 11(10)
dc.rights info:eu-repo/semantics/openAccess
dc.subject Nautgriparækt
dc.subject Ensím
dc.subject Efnagreining
dc.subject Cattle breeds
dc.subject Mass spectrometry
dc.subject Natural glycosidases
dc.subject NMR spectroscopy
dc.subject O-linked glycans
dc.subject Oligosaccharides
dc.subject Principal component analysis
dc.subject Endocrinology, Diabetes and Metabolism
dc.subject Biochemistry
dc.subject Molecular Biology
dc.title Naturally occurring glycosidases in milk from native cattle breeds : Activity and consequences on free and protein bound-glycans
dc.type /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article
dc.description.version Peer reviewed
dc.identifier.pmid 34677377
dc.identifier.doi https://doi.org/10.3390/metabo11100662
dc.relation.url http://www.scopus.com/inward/record.url?scp=85116125407&partnerID=8YFLogxK
dc.contributor.department Faculty of Food Science and Nutrition


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