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Naturally occurring glycosidases in milk from native cattle breeds : Activity and consequences on free and protein bound-glycans

Naturally occurring glycosidases in milk from native cattle breeds : Activity and consequences on free and protein bound-glycans


Title: Naturally occurring glycosidases in milk from native cattle breeds : Activity and consequences on free and protein bound-glycans
Author: Sunds, Anne Vuholm
Roland, Ida Schwartz
Sundekilde, Ulrik Kræmer
Thesbjerg, Martin Nørmark
Robinson, Randall
Bunyatratchata, Apichaya
Glantz, Maria
Paulsson, Marie
Leskauskaite, Daiva
Pihlanto, Anne
... 8 more authors Show all authors
Date: 2021-09-28
Language: English
Scope:
Department: Faculty of Food Science and Nutrition
Series: Metabolites; 11(10)
ISSN: 2218-1989
DOI: https://doi.org/10.3390/metabo11100662
Subject: Nautgriparækt; Ensím; Efnagreining; Cattle breeds; Mass spectrometry; Natural glycosidases; NMR spectroscopy; O-linked glycans; Oligosaccharides; Principal component analysis; Endocrinology, Diabetes and Metabolism; Biochemistry; Molecular Biology
URI: https://hdl.handle.net/20.500.11815/2742

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Citation:

Sunds , A V , Roland , I S , Sundekilde , U K , Thesbjerg , M N , Robinson , R , Bunyatratchata , A , Glantz , M , Paulsson , M , Leskauskaite , D , Pihlanto , A , Inglingstad , R , Devold , T G , Vegarud , G E , Birgisdóttir , B E , Guðjónsdóttir , M , Barile , D , Larsen , L B & Poulsen , N A 2021 , ' Naturally occurring glycosidases in milk from native cattle breeds : Activity and consequences on free and protein bound-glycans ' , Metabolites , vol. 11 , no. 10 , 662 . https://doi.org/10.3390/metabo11100662

Abstract:

Little is known about the extent of variation and activity of naturally occurring milk glycosidases and their potential to degrade milk glycans. A multi-omics approach was used to investigate the relationship between glycosidases and important bioactive compounds such as free oligo-saccharides and O-linked glycans in bovine milk. Using 4-methylumbelliferone (4-MU) assays activities of eight indigenous glycosidases were determined, and by mass spectrometry and1H NMR spectroscopy various substrates and metabolite products were quantified in a subset of milk samples from eight native North European cattle breeds. The results showed a clear variation in glycosidase activities among the native breeds. Interestingly, negative correlations between some glycosidases including β-galactosidase, N-acetyl-β-D-glucosaminidase, certain oligosaccharide isomers as well as O-linked glycans of κ-casein were revealed. Further, a positive correlation was found for free fucose content and α-fucosidase activity (r = 0.37, P-value < 0.001) indicating cleavage of fucosylated glycans in milk at room temperature. The results obtained suggest that milk glycosidases might partially degrade valuable glycans, which would result in lower recovery of glycans and thus represent a loss for the dairy ingredients industry if these activities are pronounced.

Description:

Funding: This research was funded by the Aarhus University Research Foundation (AUFF, Aarhus, Denmark), Frands Christian Frantsen Fund (Aarhus, Denmark), the Graduate School of Science and Technology (GSST) at Aarhus University (Aarhus, Denmark), as well as the USDA NIFA project C?−DFST-2187-H. Acknowledgments: The Aarhus University Research Foundation (AUFF, Aarhus, Denmark), Frands Christian Frantsen Fund (Aarhus, Denmark), as well as the Graduate School of Science and Technology (GSST) at Aarhus University (Aarhus, Denmark) are acknowledged for the financial support of the study. The authors from University of California, Davis are grateful for the support from the USD? NIF? project C?−DFST-2187-H. Thank you to Gitte Hald Kristiansen for helping out with the LC-MS method. We further thank the farmers and co-workers for collection of milk samples from the native breeds. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

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