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Cyclic mechanical stretch down-regulates cathelicidin antimicrobial peptide expression and activates a pro-inflammatory response in human bronchial epithelial cells

Cyclic mechanical stretch down-regulates cathelicidin antimicrobial peptide expression and activates a pro-inflammatory response in human bronchial epithelial cells


Titill: Cyclic mechanical stretch down-regulates cathelicidin antimicrobial peptide expression and activates a pro-inflammatory response in human bronchial epithelial cells
Höfundur: Káradóttir, Harpa
Nikhil Nitin, Kulkarn
Guðjónsson, Þórarinn
Karason, Sigurbergur   orcid.org/0000-0003-0726-4378
Gudmundsson, Gudmundur H
Útgáfa: 2015-12-07
Tungumál: Enska
Umfang: e1483
Háskóli/Stofnun: Háskóli Íslands
University of Iceland
Svið: Verkfræði- og náttúruvísindasvið (HÍ)
School of Engineering and Natural Sciences (UI)
Heilbrigðisvísindasvið (HÍ)
School of Health Sciences (UI)
Deild: Lífvísindasetur (HÍ)
Biomedical Center (UI)
Líf- og umhverfisvísindadeild (HÍ)
Faculty of Life and Environmental Sciences (UI)
Læknadeild (HÍ)
Faculty of Medicine (UI)
Birtist í: PeerJ;
ISSN: 2167-8359
DOI: 10.7717/peerj.1483
Efnisorð: Molecular biology; Immunology; Respiratory medicine; Sameindalíffræði; Ónæmisfræði; Öndunarfæri
URI: https://hdl.handle.net/20.500.11815/267

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Tilvitnun:

Karadottir H, Kulkarni NN, Gudjonsson T, Karason S, Gudmundsson GH. (2015) Cyclic mechanical stretch down-regulates cathelicidin antimicrobial peptide expression and activates a pro-inflammatory response in human bronchial epithelial cells. PeerJ 3:e1483 https://doi.org/10.7717/peerj.1483

Útdráttur:

Mechanical ventilation (MV) of patients can cause damage to bronchoalveolar epithelium, leading to a sterile inflammatory response, infection and in severe cases sepsis. Limited knowledge is available on the effects of MV on the innate immune defense systemin the human lung. In this study, we demonstrate that cyclic stretch of the human bronchial epithelial cell lines VA10 and BCi NS 1.1 leads to down-regulation of cathelicidin antimicrobial peptide (CAMP) gene expression. We show that treatment of VA10 cells with vitamin D3 and/or 4-phenyl butyric acid counteracted cyclic stretch mediated down-regulation of CAMP mRNA and protein expression (LL-37). Further, we observed an increase in pro-inflammatory responses in the VA10 cell line subjected to cyclic stretch. The mRNA expression of the genes encoding pro-inflammatory cytokines IL-8 and IL-1 beta was increased after cyclic stretching, where as a decrease in gene expression of chemokines IP-10 and RANTES was observed. Cyclic stretch enhanced oxidative stress in the VA10 cells. The mRNA expression of toll-like receptor (TLR) 3, TLR5 and TLR8 was reduced, while the gene expression of TLR2 was increased in VA10 cells after cyclic stretch. In conclusion, our in vitro results indicate that cyclic stretch may differentially modulate innate immunity by down-regulation of antimicrobial peptide expression and increase in pro-inflammatory responses.

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Creative Commons CC-BY 4.0

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