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Human Embryonic-Derived Mesenchymal Progenitor Cells (hES-MP Cells) are Fully Supported in Culture with Human Platelet Lysates

Human Embryonic-Derived Mesenchymal Progenitor Cells (hES-MP Cells) are Fully Supported in Culture with Human Platelet Lysates


Titill: Human Embryonic-Derived Mesenchymal Progenitor Cells (hES-MP Cells) are Fully Supported in Culture with Human Platelet Lysates
Höfundur: Jonsdottir-Buch, Sandra Mjoll   orcid.org/0000-0002-1845-5420
Gunnarsdottir, Kristbjorg
Sigurjonsson, Olafur   orcid.org/0000-0002-4968-842X
Útgáfa: 2020-07-20
Tungumál: Enska
Umfang: 75
Háskóli/Stofnun: Háskóli Íslands
University of Iceland
Háskólinn í Reykjavík
Reykjavik University
Svið: Heilbrigðisvísindasvið (HÍ)
School of Health Sciences (UI)
Tæknisvið (HR)
School of Technology (RU)
Deild: Læknadeild (HÍ)
Faculty of Medicine (UI)
Birtist í: Bioengineering;7(3)
ISSN: 2306-5354
DOI: 10.3390/bioengineering7030075
Efnisorð: Blood platelets; Cell culture techniques; Embryonic stem cells; Mesenchymal stromal cells; Progenitor cells; Fósturvísar; Stofnfrumur
URI: https://hdl.handle.net/20.500.11815/2220

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Tilvitnun:

Jonsdottir-Buch, S.M.; Gunnarsdottir, K.; Sigurjonsson, O.E. Human Embryonic-Derived Mesenchymal Progenitor Cells (hES-MP Cells) are Fully Supported in Culture with Human Platelet Lysates. Bioengineering 2020, 7, 75.

Útdráttur:

Human embryonic stem cell-derived mesenchymal progenitor (hES-MP) cells are mesenchymal-like cells, derived from human embryonic stem cells without the aid of feeder cells. They have been suggested as a potential alternative to mesenchymal stromal cells (MSCs) in regenerative medicine due to their mesenchymal-like proliferation and differentiation characteristics. Cells and cell products intended for regenerative medicine in humans should be derived, expanded and differentiated using conditions free of animal-derived products to minimize risk of animal-transmitted disease and immune reactions to foreign proteins. Human platelets are rich in growth factors needed for cell culture and have been used successfully as an animal serum replacement for MSC expansion and differentiation. In this study, we compared the proliferation of hES-MP cells and MSCs; the hES-MP cell growth was sustained for longer than that of MSCs. Growth factors, gene expression, and surface marker expression in hES-MP cells cultured with either human platelet lysate (hPL) or fetal bovine serum (FBS) supplementation were compared, along with differentiation to osteogenic and chondrogenic lineages. Despite some differences between hES-MP cells grown in hPL- and FBS-supplemented media, hPL was found to be a suitable replacement for FBS. In this paper, we demonstrate for the first time that hES-MP cells can be grown using platelet lysates from expired platelet concentrates (hPL). View Full-Text

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

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