dc.contributor |
Háskóli Íslands |
dc.contributor |
University of Iceland |
dc.contributor.author |
Juliusson, Haraldur Y. |
dc.contributor.author |
Sigurdsson, Snorri |
dc.date.accessioned |
2020-08-11T15:40:12Z |
dc.date.available |
2020-08-11T15:40:12Z |
dc.date.issued |
2020-07 |
dc.identifier.citation |
Juliusson, H. Y., & Sigurdsson, S. T. (2020). Nitroxide-derived N-oxide phenazines for noncovalent spin-labeling of DNA. ChemBioChem, doi: 10.1002/cbic.202000128 |
dc.identifier.issn |
1439-4227 |
dc.identifier.issn |
1439-7633 (eISSN) |
dc.identifier.uri |
https://hdl.handle.net/20.500.11815/1975 |
dc.description |
Post-print (lokagerð höfundar) |
dc.description.abstract |
Two o‐benzoquinone derivatives of isoindoline were synthesized for use as building blocks to incorporate isoindoline nitroxides into different compounds and materials. These o‐quinones were condensed with a number of o‐phenylenediamines to form isoindoline‐phenazines in high yields. Subsequent oxidation gave phenazine‐di‐N‐oxide isoindoline nitroxides that were evaluated for noncovalent and site‐directed spin‐labeling of duplex DNA and RNA that contained abasic sites. Although only minor binding was observed for RNA, the unsubstituted phenazine‐N,N‐dioxide tetramethyl isoindoline nitroxide showed high binding affinity and selectivity towards abasic sites in duplex DNA that contained cytosine as the orphan base. |
dc.description.sponsorship |
The authors acknowledge financial support from the Icelandic Research Fund (173727-051). The authors thank Dr S. Jonsdottir for assistance with collecting analytical data for structural characterization of new compounds, Dr. Thomas Halbritter for critically reading this manuscript, and the members of the Sigurdsson research group for helpful discussions. |
dc.language.iso |
en |
dc.publisher |
Wiley |
dc.rights |
info:eu-repo/semantics/embargoedAccess |
dc.subject |
Aminoxyl radicals |
dc.subject |
EPR spectroscopy |
dc.subject |
Noncovalent spin labels |
dc.subject |
Oligonucleotides |
dc.subject |
Phenazines |
dc.subject |
Litrófsgreining |
dc.subject |
Sameindaerfðafræði |
dc.title |
Nitroxide‐Derived N‐Oxide Phenazines for Noncovalent Spin‐Labeling of DNA |
dc.type |
info:eu-repo/semantics/article |
dcterms.license |
This is the peer reviewed version of the following article: [Juliusson, H. Y., & Sigurdsson, S. T. (2020). Nitroxide-derived N-oxide phenazines for noncovalent spin-labeling of DNA. ChemBioChem, doi: 10.1002/cbic.202000128], which has been published in final form at https://doi.org/10.1002/cbic.202000128. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. |
dc.description.version |
Peer reviewed |
dc.identifier.journal |
ChemBioChem |
dc.identifier.doi |
10.1002/cbic.202000128 |
dc.contributor.department |
Raunvísindadeild (HÍ) |
dc.contributor.department |
Faculty of Physical Sciences (UI) |
dc.contributor.department |
Raunvísindastofnun (HÍ) |
dc.contributor.department |
Science Institute (UI) |
dc.contributor.school |
Verkfræði- og náttúruvísindasvið (HÍ) |
dc.contributor.school |
School of Engineering and Natural Sciences (UI) |