dc.contributor |
Háskóli Íslands |
dc.contributor |
University of Iceland |
dc.contributor.author |
Gophane, Dnyaneshwar B. |
dc.contributor.author |
Endeward, Burkhard |
dc.contributor.author |
Prisner, Thomas F. |
dc.contributor.author |
Sigurdsson, Snorri |
dc.date.accessioned |
2020-08-10T15:31:12Z |
dc.date.available |
2020-08-10T15:31:12Z |
dc.date.issued |
2018 |
dc.identifier.citation |
Gophane, D. B., Endeward, B., Prisner, T. F., & Sigurdsson, S. T. (2018). A semi-rigid isoindoline-derived nitroxide spin label for RNA. Organic and Biomolecular Chemistry, 16(5), 816-824 doi: 10.1039/c7ob02870a |
dc.identifier.issn |
1477-0520 |
dc.identifier.issn |
1477-0539 (eISSN) |
dc.identifier.uri |
https://hdl.handle.net/20.500.11815/1962 |
dc.description |
Post-print (lokagerð höfundar) |
dc.description.abstract |
A new isoindoline-derived benzimidazole nitroxide spin label, ImUm, was synthesized and incorporated into RNA oligoribonucleotides. ImUm is the first example of a conformationally unambiguous spin label for RNA, in which the nitroxide N–O bond lies on the same axis as the single bond used to attach the rigid isoindoline-based spin label to a uridine base. This results in minimal displacement of the nitroxide upon rotation of this single bond, which is a useful property for a label to be used for distance measurements. Continuous-wave (CW) EPR measurements of RNA duplexes containing ImUm indicate a restricted rotation around this single bond, presumably due to an intramolecular hydrogen bond between the benzimidazole N–H and O4 of the uracil. Orientation-selective pulsed electron–electron double resonance (PELDOR, also called double electron–electron resonance, or DEER) distance measurements between two spin labels in two RNA duplexes showed in one case a strong orientation dependence, further confirming the restricted motion of the spin labels in RNA duplexes. |
dc.description.sponsorship |
S. Th. S. acknowledges financial support from the Icelandic Research Fund (141062-051). T. F. P. acknowledges financial support by the SFB 902 - Molecular Principles of RNA-based Regulation. We thank Dr S. Jonsdottir for assistance in collecting analytical data for structural characterization of the new compounds and Dr Subham Saha for assistance with preparation of this manuscript. |
dc.format.extent |
816-824 |
dc.language.iso |
en |
dc.publisher |
Royal Society of Chemistry (RSC) |
dc.relation.ispartofseries |
Organic & Biomolecular Chemistry;16(5) |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.subject |
DNA-rannsóknir |
dc.subject |
Kjarnsýrur |
dc.subject |
Rafeindir |
dc.title |
A semi-rigid isoindoline-derived nitroxide spin label for RNA |
dc.type |
info:eu-repo/semantics/article |
dc.description.version |
Peer reviewed |
dc.identifier.journal |
Organic & Biomolecular Chemistry |
dc.identifier.doi |
10.1039/C7OB02870A |
dc.relation.url |
http://pubs.rsc.org/en/content/articlepdf/2018/OB/C7OB02870A |
dc.contributor.department |
Raunvísindadeild (HÍ) |
dc.contributor.department |
Faculty of Physical Sciences (UI) |
dc.contributor.department |
Raunvísindastofnun (HÍ) |
dc.contributor.department |
Science Institute (UI) |
dc.contributor.school |
Verkfræði- og náttúruvísindasvið (HÍ) |
dc.contributor.school |
School of Engineering and Natural Sciences (UI) |