dc.contributor |
Háskóli Íslands |
dc.contributor |
University of Iceland |
dc.contributor.author |
Jagtap, A. P. |
dc.contributor.author |
Krstic, I. |
dc.contributor.author |
Kunjir, N. C. |
dc.contributor.author |
Hänsel, R. |
dc.contributor.author |
Prisner, Thomas F. |
dc.contributor.author |
Sigurdsson, Snorri |
dc.date.accessioned |
2020-08-10T13:58:43Z |
dc.date.available |
2020-08-10T13:58:43Z |
dc.date.issued |
2014-11-20 |
dc.identifier.citation |
A. P. Jagtap, I. Krstic, N. C. Kunjir, R. Hänsel, T. F. Prisner & S. Th. Sigurdsson (2015) Sterically shielded spin labels for in-cell EPR spectroscopy: Analysis of stability in reducing environment, Free Radical Research, 49:1, 78-85DOI: 10.3109/10715762.2014.979409 |
dc.identifier.issn |
1071-5762 |
dc.identifier.issn |
1029-2470 (eISSN) |
dc.identifier.uri |
https://hdl.handle.net/20.500.11815/1954 |
dc.description |
Post-print (lokagerð höfundar) |
dc.description.abstract |
Electron paramagnetic resonance (EPR) spectroscopy is a powerful and widely used technique for studying structure and dynamics of biomolecules under bio-orthogonal conditions. In-cell EPR is an emerging area in this field; however, it is hampered by the reducing environment present in cells, which reduces most nitroxide spin labels to their corresponding diamagnetic N-hydroxyl derivatives. To determine which radicals are best suited for in-cell EPR studies, we systematically studied the effects of substitution on radical stability using five different classes of radicals, specifically piperidine-, imidazolidine-, pyrrolidine-, and isoindoline-based nitroxides as well as the Finland trityl radical. Thermodynamic parameters of nitroxide reduction were determined by cyclic voltammetry; the rate of reduction in the presence of ascorbate, cellular extracts, and after injection into oocytes was measured by continuous-wave EPR spectroscopy. Our study revealed that tetraethyl-substituted nitroxides are good candidates for in-cell EPR studies, in particular pyrrolidine derivatives, which are slightly more stable than the trityl radical. |
dc.description.sponsorship |
This work was supported by the Icelandic Research Fund (120001021), the Deutsche Forschungsgemeinschaft (SFB 902, Molecular principles of RNA-based regulation) and by a doctoral fellowship to A. P. Jagtap from the University of Iceland Research Fund. |
dc.format.extent |
78-85 |
dc.language.iso |
en |
dc.publisher |
Informa UK Limited |
dc.relation.ispartofseries |
Free Radical Research;49(1) |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.subject |
Nitroxide reduction |
dc.subject |
Aminoxyl radical |
dc.subject |
Radical stability |
dc.subject |
Spin labeling |
dc.subject |
Trityl radical |
dc.subject |
Litrófsgreining |
dc.subject |
Efnagreining |
dc.title |
Sterically shielded spin labels for in-cell EPR spectroscopy: Analysis of stability in reducing environment |
dc.type |
info:eu-repo/semantics/article |
dcterms.license |
This is an Accepted Manuscript of an article published by Taylor & Francis in Free Radical Research on 20. nov 2014, available online: http://www.tandfonline.com/10.3109/10715762.2014.979409. |
dc.description.version |
Peer reviewed |
dc.identifier.journal |
Free Radical Research |
dc.identifier.doi |
10.3109/10715762.2014.979409 |
dc.relation.url |
http://www.tandfonline.com/doi/pdf/10.3109/10715762.2014.979409 |
dc.contributor.department |
Raunvísindadeild (HÍ) |
dc.contributor.department |
Faculty of Physical Sciences (UI) |
dc.contributor.department |
Raunvísindastofnun (HÍ) |
dc.contributor.department |
Science Institute (UI) |
dc.contributor.school |
Verkfræði- og náttúruvísindasvið (HÍ) |
dc.contributor.school |
School of Engineering and Natural Sciences (UI) |