dc.contributor |
Háskóli Íslands |
dc.contributor |
University of Iceland |
dc.contributor.author |
Gophane, Dnyaneshwar B. |
dc.contributor.author |
Endeward, Burkhard |
dc.contributor.author |
Prisner, Thomas F. |
dc.contributor.author |
Sigurdsson, Snorri |
dc.date.accessioned |
2020-08-10T13:54:20Z |
dc.date.available |
2020-08-10T13:54:20Z |
dc.date.issued |
2014-10-08 |
dc.identifier.citation |
Gophane, D.B., Endeward, B., Prisner, T.F. and Sigurdsson, S.T. (2014), Conformationally Restricted Isoindoline‐Derived Spin Labels in Duplex DNA: Distances and Rotational Flexibility by Pulsed Electron–Electron Double Resonance Spectroscopy. Chem. Eur. J., 20: 15913-15919. doi:10.1002/chem.201403726 |
dc.identifier.issn |
0947-6539 |
dc.identifier.issn |
1521-3765 (eISSN) |
dc.identifier.uri |
https://hdl.handle.net/20.500.11815/1953 |
dc.description |
Post-print (lokagerð höfundar) |
dc.description.abstract |
Three structurally related isoindoline-derived spin labels that have different mobilities were incorporated into duplex DNA to systematically study the effect of motion on orientation-dependent pulsed electron-electron double resonance (PELDOR) measurements. To that end, a new nitroxide spin label, U-ExIm, was synthesized and incorporated into DNA oligonucleotides. U-ExIm is the first example of a conformationally unambiguous spin label for nucleic acids, in which the nitroxide N-O bond lies on the same axis as the three single bonds used to attach the otherwise rigid isoindoline- based spin label to a uridine base. Continuous-wave (CW) EPR measurements of U-ExIm confirm a very high rotational mobility of the spin label in duplex DNA relative to the structurally related spin label U-Im, which has restricted mobility due to an intramolecular hydrogen bond. The Xband CW-EPR spectra of U-ExIm can be used to identify mismatches in duplex DNA. PELDOR distance measurements between pairs of the spin labels U-Im, OxU, and U-ExIm in duplex DNA showed a strong angular dependence for U-Im, a medium dependence for OxU, and no orientation effect for U-ExIm. Thus, precise distances can be extracted from U-ExIm without having to take orientational effects into account. |
dc.description.sponsorship |
S.Th.S. acknowledges financial support from the Icelandic Research Fund (080041023 and 120001021). T. F. P. acknowledges financial support from the German Research Foundation (CRC902: Molecular Principles of RNA-Based Regulation). We thank H. Gustmann and P. Wenk for help in collecting the PELDOR data, Dr. G. Reginsson for simulations of CW-EPR spectra, and Dr. S. Jonsdottir for assistance in collecting analytical data for structural characterization of the compounds that were prepared. |
dc.format.extent |
15913-15919 |
dc.language.iso |
en |
dc.publisher |
Wiley |
dc.relation.ispartofseries |
Chemistry - A European Journal;20(48) |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.subject |
EPR Spectroscopy |
dc.subject |
Nucleic acids |
dc.subject |
PELDOR |
dc.subject |
DEER |
dc.subject |
Aminoxyl radical |
dc.subject |
Litrófsgreining |
dc.subject |
Kjarnsýrur |
dc.title |
Conformationally Restricted Isoindoline-Derived Spin Labels in Duplex DNA: Distances and Rotational Flexibility by Pulsed Electron-Electron Double Resonance Spectroscopy |
dc.type |
info:eu-repo/semantics/article |
dc.description.version |
Peer reviewed |
dc.identifier.journal |
Chemistry - A European Journal |
dc.identifier.doi |
10.1002/chem.201403726 |
dc.contributor.department |
Raunvísindadeild (HÍ) |
dc.contributor.department |
Faculty of Physical Sciences (UI) |
dc.contributor.school |
Verkfræði- og náttúruvísindasvið (HÍ) |
dc.contributor.school |
School of Engineering and Natural Sciences (UI) |