Opin vísindi

A standalone bioreactor system to deliver compressive load under perfusion flow to hBMSC-seeded 3D chitosan-graphene templates

A standalone bioreactor system to deliver compressive load under perfusion flow to hBMSC-seeded 3D chitosan-graphene templates


Title: A standalone bioreactor system to deliver compressive load under perfusion flow to hBMSC-seeded 3D chitosan-graphene templates
Author: Lovecchio, Joseph   orcid.org/0000-0002-4721-1388
Gargiulo, Paolo   orcid.org/0000-0002-5049-4817
Vargas Luna, José Luis   orcid.org/0000-0002-5125-1999
Giordano, Emanuele
Sigurjónsson, Ólafur Eysteinn
Date: 2019-11-14
Language: English
Scope: 16854
University/Institute: Háskólinn í Reykjavík
Reykjavik University
School: Tæknisvið (HR)
School of Technology (RU)
Department: Verkfræðideild (HR)
Department of Engineering (RU)
Series: Scientific Reports;9(1)
ISSN: 2045-2322
DOI: 10.1038/s41598-019-53319-7
Subject: Biomedical engineering; Tissue engineering; Stem cells; Cell culture; Bone marrow; Extracellular matrix; Chitosan; Graphene; Scaffold; Three-dimension; Verkfræði; Vefjafræði; Lífverkfræði; Stofnfrumur; Beinmergur; Frumuræktun; Prótín; Kítósan; Þrívídd
URI: https://hdl.handle.net/20.500.11815/1858

Show full item record

Citation:

Lovecchio, J., Gargiulo, P., Luna, J. L. V., Giordano, E., & Sigurjonsson, O. E. (2019). A standalone bioreactor system to deliver compressive load under perfusion flow to hBMSC-seeded 3D chitosan-graphene templates. Scientific Reports, 9, 16854. https://doi.org/10.1038/s41598-019-53319-7

Abstract:

The availability of engineered biological tissues holds great potential for both clinical applications and basic research in a life science laboratory. A prototype standalone perfusion/compression bioreactor system was proposed to address the osteogenic commitment of stem cells seeded onboard of 3D chitosan-graphene (CHT/G) templates. Testing involved the coordinated administration of a 1 mL/min medium flow rate together with dynamic compression (1% strain at 1 Hz; applied twice daily for 30 min) for one week. When compared to traditional static culture conditions, the application of perfusion and compression stimuli to human bone marrow stem cells using the 3D CHT/G template scaffold induced a sizable effect. After using the dynamic culture protocol, there was evidence of a larger number of viable cells within the inner core of the scaffold and of enhanced extracellular matrix mineralization. These observations show that our novel device would be suitable for addressing and investigating the osteogenic phenotype commitment of stem cells, for both potential clinical applications and basic research.

Description:

Publisher's version (útgefin grein)

Rights:

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Files in this item

This item appears in the following Collection(s)