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Peptidylarginine deiminase and deiminated proteins are detected throughout early halibut ontogeny - Complement components C3 and C4 are post-translationally deiminated in halibut (Hippoglossus hippoglossus L.)

Peptidylarginine deiminase and deiminated proteins are detected throughout early halibut ontogeny - Complement components C3 and C4 are post-translationally deiminated in halibut (Hippoglossus hippoglossus L.)


Title: Peptidylarginine deiminase and deiminated proteins are detected throughout early halibut ontogeny - Complement components C3 and C4 are post-translationally deiminated in halibut (Hippoglossus hippoglossus L.)
Author: Magnadottir, Bergljot   orcid.org/0000-0002-5737-2345
Bragason, Birkir   orcid.org/0000-0002-3962-8036
Bricknell, Ian   orcid.org/0000-0001-6042-7143
Bowden, Timothy   orcid.org/0000-0002-2295-1179
Nicholas, Anthony P.
Hristova, Mariya
Guðmundsdóttir, Sigríður   orcid.org/0000-0003-4645-0229
Dodds, Alister W.
Lange, Sigrun
Date: 2019-03
Language: English
Scope: 1-19
University/Institute: Háskóli Íslands
University of Iceland
Department: Tilraunastöð í meinafræði að Keldum (HÍ)
Institute for Experimental Pathology, Keldur (UI)
Series: Developmental & Comparative Immunology;92
ISSN: 0145-305X
DOI: 10.1016/j.dci.2018.10.016
Subject: Complement; Halibut; Hippoglossus hippoglossus L; Ontogeny; Pentraxin; Peptidylarginine deiminase; Protein deimination; Lúða; Lífeðlisfræði; Meinafræði; Prótín
URI: https://hdl.handle.net/20.500.11815/1802

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Citation:

Magnadóttir, B. et al., 2019. Peptidylarginine deiminase and deiminated proteins are detected throughout early halibut ontogeny - Complement components C3 and C4 are post-translationally deiminated in halibut (Hippoglossus hippoglossus L.). Developmental and Comparative Immunology, 92, pp.1–19.

Abstract:

Post-translational protein deimination is mediated by peptidylarginine deiminases (PADs), which are calcium dependent enzymes conserved throughout phylogeny with physiological and pathophysiological roles. Protein deimination occurs via the conversion of protein arginine into citrulline, leading to structural and functional changes in target proteins. In a continuous series of early halibut development from 37 to 1050° d, PAD, total deiminated proteins and deiminated histone H3 showed variation in temporal and spatial detection in various organs including yolksac, muscle, skin, liver, brain, eye, spinal cord, chondrocytes, heart, intestines, kidney and pancreas throughout early ontogeny. For the first time in any species, deimination of complement components C3 and C4 is shown in halibut serum, indicating a novel mechanism of complement regulation in immune responses and homeostasis. Proteomic analysis of deiminated target proteins in halibut serum further identified complement components C5, C7, C8 C9 and C1 inhibitor, as well as various other immunogenic, metabolic, cytoskeletal and nuclear proteins. Post-translational deimination may facilitate protein moonlighting, an evolutionary conserved phenomenon, allowing one polypeptide chain to carry out various functions to meet functional requirements for diverse roles in immune defences and tissue remodelling.

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/)

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