dc.contributor |
Háskóli Íslands |
dc.contributor |
University of Iceland |
dc.contributor.author |
Smid, Marcel |
dc.contributor.author |
Wilting, Saskia M. |
dc.contributor.author |
Uhr, Katharina |
dc.contributor.author |
Rodríguez-González, F. Germán |
dc.contributor.author |
de Weerd, Vanja |
dc.contributor.author |
Prager-Van der Smissen, Wendy J.C. |
dc.contributor.author |
van der Vlugt-Daane, Michelle |
dc.contributor.author |
van Galen, Anne |
dc.contributor.author |
Nik-Zainal, Serena |
dc.contributor.author |
Butler, Adam |
dc.contributor.author |
Martin, Sancha |
dc.contributor.author |
Davies, Helen R. |
dc.contributor.author |
Staaf, Johan |
dc.contributor.author |
van de Vijver, Marc J. |
dc.contributor.author |
Richardson, Andrea L. |
dc.contributor.author |
MacGrogan, Gaëten |
dc.contributor.author |
Salgado, Roberto |
dc.contributor.author |
van den Eynden, Gert G.G.M. |
dc.contributor.author |
Purdie, Colin A. |
dc.contributor.author |
Thompson, Alastair M. |
dc.contributor.author |
Caldas, Carlos |
dc.contributor.author |
Span, Paul N. |
dc.contributor.author |
Sweep, Fred C.G.J. |
dc.contributor.author |
Simpson, Peter T. |
dc.contributor.author |
Lakhani, Sunil R. |
dc.contributor.author |
Van Laere, Steven |
dc.contributor.author |
Desmedt, Christine |
dc.contributor.author |
Paradiso, Angelo |
dc.contributor.author |
Eyfjörð, Jórunn Erla |
dc.contributor.author |
Broeks, Annegien |
dc.contributor.author |
Vincent-Salomon, Anne |
dc.contributor.author |
Futreal, Andrew P. |
dc.contributor.author |
Knappskog, Stian |
dc.contributor.author |
King, Tari |
dc.contributor.author |
Viari, Alain |
dc.contributor.author |
Børresen-Dale, Anne-Lise |
dc.contributor.author |
Stunnenberg, Hendrik G. |
dc.contributor.author |
Stratton, Mike |
dc.contributor.author |
Foekens, John A. |
dc.contributor.author |
Sieuwerts, Anieta M. |
dc.contributor.author |
Martens, John W.M. |
dc.date.accessioned |
2020-05-14T14:26:33Z |
dc.date.available |
2020-05-14T14:26:33Z |
dc.date.issued |
2019-01-28 |
dc.identifier.citation |
Smid et al., 2019. The circular RNome of primary breast cancer. Genome research, 29(3), pp.356–366. |
dc.identifier.issn |
1088-9051 |
dc.identifier.issn |
1549-5469 (eISSN) |
dc.identifier.uri |
https://hdl.handle.net/20.500.11815/1797 |
dc.description |
Publisher's version (útgefin grein) |
dc.description.abstract |
Circular RNAs (circRNAs) are a class of RNAs that is under increasing scrutiny, although their functional roles are debated. We analyzed RNA-seq data of 348 primary breast cancers and developed a method to identify circRNAs that does not rely on unmapped reads or known splice junctions. We identified 95,843 circRNAs, of which 20,441 were found recurrently. Of the circRNAs that match exon boundaries of the same gene, 668 showed a poor or even negative (R < 0.2) correlation with the expression level of the linear gene. In silico analysis showed only a minority (8.5%) of circRNAs could be explained by known splicing events. Both these observations suggest that specific regulatory processes for circRNAs exist. We confirmed the presence of circRNAs of CNOT2, CREBBP, and RERE in an independent pool of primary breast cancers. We identified circRNA profiles associated with subgroups of breast cancers and with biological and clinical features, such as amount of tumor lymphocytic infiltrate and proliferation index. siRNA-mediated knockdown of circCNOT2 was shown to significantly reduce viability of the breast cancer cell lines MCF-7 and BT-474, further underlining the biological relevance of circRNAs. Furthermore, we found that circular, and not linear, CNOT2 levels are predictive for progression-free survival time to aromatase inhibitor (AI) therapy in advanced breast cancer patients, and found that circCNOT2 is detectable in cell-free RNA from plasma. We showed that circRNAs are abundantly present, show characteristics of being specifically regulated, are associated with clinical and biological properties, and thus are relevant in breast cancer. |
dc.description.sponsorship |
We thank the Erasmus MC Cancer Computational Biology Center for giving access to their IT infrastructure and the software that was used for the computations and data analysis in this study. We thank Sandra Albassam for her help with the first versions of the script to identify circular regions. We thank Maurice P.H.M. Jansen, Jean C. Helmijr, Inge de Kruijff, and Manouk K. Bos for their help in evaluating plasma samples that were gathered in the EU-FP7 CareMore (nr 601760) project. We thank for technical support Miriam Ragle Aure and Anita Langerød of the Oslo University Hospital, Norway; Ewan Birney of the European Bioinformatics Institute, UK; and Stefania Tommasi of the IRCCS Istituto Tumori “Giovanni Paolo II,” Bari, Italy. We thank the Oslo Breast Cancer Research Consortium (OSBREAC), Norway (https://www.ous-research.no/home/kgjebsen/home/14105) for contributing patient samples and Sabine Linn and Marleen Kok of The Netherlands Cancer Institute for contributing samples for the AI cohort. Finally, we thank all members of the ICGC Breast Cancer Working Group. This work has been funded through the ICGC Breast Cancer Working group by the Breast Cancer Somatic Genetics Study (a European research project funded by the European Community’s Seventh Framework Programme (FP7/2010-2014) under the grant agreement number 242006) and the Triple Negative project funded by the Wellcome Trust (grant reference 077012/Z/05/Z). F.G.R.-G. and S.M. were funded by BASIS. J.A.F. was funded through an ERC Advanced Grant (ERC-2012-AdG-322737) and ERC Proof-of-Concept Grant (ERC-2017-PoC-767854). K.U. was funded by the Daniel den Hoed Foundation. S.N.-Z. is a Wellcome Beit Fellow and personally funded by a Wellcome Trust Intermediate Fellowship (WT100183MA). A.L.R. is partially supported by the Dana-Farber/Harvard Cancer Center SPORE in Breast Cancer (NIH/NCI 5 P50 CA16 8504-02). A.M.S. was supported by Cancer Genomics Netherlands (CGC.nl) through a grant from the Netherlands Organization of Scientific research (NWO). M. Smid was supported by the EU-FP7-DDR response project. C.D. was supported by a grant from the Breast Cancer Research Foundation. J.E. was funded by The Icelandic Centre for Research (RANNIS). |
dc.format.extent |
356-366 |
dc.language.iso |
en |
dc.publisher |
Cold Spring Harbor Laboratory |
dc.relation |
info:eu-repo/grantAgreement/EC/FP7/601760 |
dc.relation.ispartofseries |
Genome Research;29(3) |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.subject |
Genetics |
dc.subject |
Breast cancer |
dc.subject |
RNAs |
dc.subject |
Brjóstakrabbamein |
dc.subject |
Erfðafræði |
dc.subject |
DNA-rannsóknir |
dc.title |
The circular RNome of primary breast cancer |
dc.type |
info:eu-repo/semantics/article |
dcterms.license |
This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/. |
dc.description.version |
Peer Reviewed |
dc.identifier.journal |
Genome Research |
dc.identifier.doi |
10.1101/gr.238121.118 |
dc.relation.url |
https://syndication.highwire.org/content/doi/10.1101/gr.238121.118 |
dc.contributor.department |
Læknadeild (HÍ) |
dc.contributor.department |
Faculty of Medicine (UI) |
dc.contributor.school |
Heilbrigðisvísindasvið (HÍ) |
dc.contributor.school |
School of Health Sciences (UI) |