dc.contributor.author |
Nguyen, Hang Thi |
dc.contributor.author |
Bao, Huynh Nguyen Duy |
dc.contributor.author |
Dang, Huong Thi Thu |
dc.contributor.author |
Tómasson, Tumi |
dc.contributor.author |
Arason, Sigurjón |
dc.contributor.author |
Guðjónsdóttir, María |
dc.date.accessioned |
2023-08-15T01:05:35Z |
dc.date.available |
2023-08-15T01:05:35Z |
dc.date.issued |
2022-12 |
dc.identifier.citation |
Nguyen , H T , Bao , H N D , Dang , H T T , Tómasson , T , Arason , S & Guðjónsdóttir , M 2022 , ' Protein Characteristics and Bioactivity of Fish Protein Hydrolysates from Tra Catfish (Pangasius hypophthalmus) Side Stream Isolates ' , Foods , vol. 11 , no. 24 , 4102 . https://doi.org/10.3390/foods11244102 |
dc.identifier.issn |
2304-8158 |
dc.identifier.other |
75772975 |
dc.identifier.other |
83304925-6b60-4484-88e0-c0b84a072c8d |
dc.identifier.other |
85144691214 |
dc.identifier.uri |
https://hdl.handle.net/20.500.11815/4399 |
dc.description |
Funding Information: This research was supported by the UNESCO GRÓ−Fisheries Training Programme and The Icelandic Food Research Fund in Iceland (Matvælasjóður). Publisher Copyright: © 2022 by the authors. |
dc.description.abstract |
Enzymatic hydrolysis is a novel method to recover highly potent bioactive fish protein hydrolysates (FPHs) from fish processing side-streams. The common way of producing FPHs directly from fish side-streams may be inappropriate due to the excess of lipids and pro-oxidants, especially in lipid-rich streams, as obtained from Tra catfish. This study aimed to optimise the hydrolysis conditions for a commercial enzyme (Alcalase® 2.4 L) (enzyme concentrate, temperature, and time) in FPH production from the fish protein isolate obtained from Tra catfish dark muscle (DM-FPI) using the pH-shift method. The degree of hydrolysis (DH), protein recovery (PR), and antioxidant properties, including DPPH radical scavenging activity (DPPH-RSA) and total reducing power capacity (TRPC), were measured to evaluate the effects of the hydrolysis conditions on the FPHs. Optimal hydrolysis was obtained at an enzyme/substrate protein ratio of 3% (v/w) and a hydrolysis temperature of 50 °C for 3 h. The FPHs obtained from different substrates, including DM-FPI, abdominal cut-off (ACO) FPI, and head and backbone blend (HBB) FPI, had similar DHs under these optimum conditions, ranging from 22.5% to 24.0%. However, the FPH obtained from abdominal cut-off isolate (ACO-FPH) showed the highest PR of 81.5 ± 4.3% and the highest antioxidant properties, with a DPPH-RSA of 86.1 ± 1.6% and a TRPC of 6.4 ± 0.4 equivalent mg vitamin C/g protein. The resulting FPHs present a natural source of antioxidants with great potential for food applications, especially the ACO-FPH. In addition, all FPHs had excellent amino acid profiles, indicating strong potential for their use as supplements. Tra catfish protein-rich side-streams can thus be processed into high-value bioactive FPHs using Alcalase for human consumption. |
dc.format.extent |
1747347 |
dc.format.extent |
|
dc.language.iso |
en |
dc.relation.ispartofseries |
Foods; 11(24) |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.subject |
amino acids |
dc.subject |
antioxidant |
dc.subject |
dark muscle |
dc.subject |
enzymatic hydrolysis |
dc.subject |
fish protein isolates |
dc.subject |
side streams |
dc.subject |
Food Science |
dc.subject |
Microbiology |
dc.subject |
Health (social science) |
dc.subject |
Health Professions (miscellaneous) |
dc.subject |
Plant Science |
dc.title |
Protein Characteristics and Bioactivity of Fish Protein Hydrolysates from Tra Catfish (Pangasius hypophthalmus) Side Stream Isolates |
dc.type |
/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article |
dc.description.version |
Peer reviewed |
dc.identifier.doi |
10.3390/foods11244102 |
dc.relation.url |
http://www.scopus.com/inward/record.url?scp=85144691214&partnerID=8YFLogxK |
dc.contributor.department |
Faculty of Food Science and Nutrition |