Opin vísindi

The cerebrospinal fluid proteome of preterm infants predicts neurodevelopmental outcome

The cerebrospinal fluid proteome of preterm infants predicts neurodevelopmental outcome

Title: The cerebrospinal fluid proteome of preterm infants predicts neurodevelopmental outcome
Author: Leifsdottir, Kristin
Jost, Kerstin
Siljehav, Veronica
Thelin, Eric P.
Lassarén, Philipp
Nilsson, Peter
Haraldsson, Ásgeir
Eksborg, Staffan
Herlenius, Eric
Date: 2022-07-19
Language: English
Scope: 1372940
Department: Women's and Childrens's Services
Faculty of Medicine
Series: Frontiers in Pediatrics; 10()
ISSN: 2296-2360
DOI: 10.3389/fped.2022.921444
Subject: cerebrospinal fluid; neonatal sepsis; neurodevelopmental outcome; outcome prediction; preterm infant; proteomic profile; Pediatrics, Perinatology and Child Health
URI: https://hdl.handle.net/20.500.11815/3799

Show full item record


Leifsdottir , K , Jost , K , Siljehav , V , Thelin , E P , Lassarén , P , Nilsson , P , Haraldsson , Á , Eksborg , S & Herlenius , E 2022 , ' The cerebrospinal fluid proteome of preterm infants predicts neurodevelopmental outcome ' , Frontiers in Pediatrics , vol. 10 , 921444 , pp. 921444 . https://doi.org/10.3389/fped.2022.921444


Background: Survival rate increases for preterm infants, but long-term neurodevelopmental outcome predictors are lacking. Our primary aim was to determine whether a specific proteomic profile in cerebrospinal fluid (CSF) of preterm infants differs from that of term infants and to identify novel biomarkers of neurodevelopmental outcome in preterm infants. Methods: Twenty-seven preterm infants with median gestational age 27 w + 4 d and ten full-term infants were enrolled prospectively. Protein profiling of CSF were performed utilizing an antibody suspension bead array. The relative levels of 178 unique brain derived proteins and inflammatory mediators, selected from the Human Protein Atlas, were measured. Results: The CSF protein profile of preterm infants differed from that of term infants. Increased levels of brain specific proteins that are associated with neurodevelopment and neuroinflammatory pathways made up a distinct protein profile in the preterm infants. The most significant differences were seen in proteins involved in neurodevelopmental regulation and synaptic plasticity, as well as components of the innate immune system. Several proteins correlated with favorable outcome in preterm infants at 18–24 months corrected age. Among the proteins that provided strong predictors of outcome were vascular endothelial growth factor C, Neurocan core protein and seizure protein 6, all highly important in normal brain development. Conclusion: Our data suggest a vulnerability of the preterm brain to postnatal events and that alterations in protein levels may contribute to unfavorable neurodevelopmental outcome.


Funding Information: This study was funded by the Karolinska Institutet, the University Hospital of Iceland and the Swedish Society for Medical Research, the Swedish Brain Foundation (FO2019-0087 and FO2019-0006), Strategic Research Area Neuroscience (StratNeuro), Ehrling-Person Family Foundation, Axel Tielmans, Freemasons Children’s House, the Swedish National Heart and Lung (20180505) Foundations, the Swedish Research Council (2019-01157), and the Stockholm County Council (20190400). KJ received funding from the Swiss National Science Foundation (Postdoc Mobility Fellowship, P400PM_194474. The funders did not participate in the design or conduct of the study. Publisher Copyright: Copyright © 2022 Leifsdottir, Jost, Siljehav, Thelin, Lassarén, Nilsson, Haraldsson, Eksborg and Herlenius.

Files in this item

This item appears in the following Collection(s)