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What does soil-transmitted helminth elimination look like? Results from a targeted molecular detection survey in Japan

What does soil-transmitted helminth elimination look like? Results from a targeted molecular detection survey in Japan


Title: What does soil-transmitted helminth elimination look like? Results from a targeted molecular detection survey in Japan
Author: Hasegawa, Mitsuko
Pilotte, Nils
Kikuchi, Mihoko
Means, Arianna R.
Papaiakovou, Marina
Gonzalez, Andrew M.
Maasch, Jacqueline R.M.A.
Ikuno, Hiroshi
Sunahara, Toshihiko
Ásbjörnsdóttir, Kristjana H.
... 3 more authors Show all authors
Date: 2020-01-08
Language: English
Scope:
Department: Faculty of Medicine
Series: Parasites and Vectors; 13(1)
ISSN: 1756-3305
DOI: https://doi.org/10.1186/s13071-019-3875-z
Subject: Ancylostoma duodenale; Ascaris lumbricoides; Multi-parallel real-time PCR; Necator americanus; Soil-transmitted helminth; STH; Targeted prevalence survey; Trichuris trichiura; WASH; Parasitology; Infectious Diseases
URI: https://hdl.handle.net/20.500.11815/3391

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Citation:

Hasegawa , M , Pilotte , N , Kikuchi , M , Means , A R , Papaiakovou , M , Gonzalez , A M , Maasch , J R M A , Ikuno , H , Sunahara , T , Ásbjörnsdóttir , K H , Walson , J L , Williams , S A & Hamano , S 2020 , ' What does soil-transmitted helminth elimination look like? Results from a targeted molecular detection survey in Japan ' , Parasites and Vectors , vol. 13 , no. 1 , 6 . https://doi.org/10.1186/s13071-019-3875-z

Abstract:

Background: Japan is one of the few countries believed to have eliminated soil-transmitted helminths (STHs). In 1949, the national prevalence of Ascaris lumbricoides was 62.9%, which decreased to 0.6% in 1973 due to improvements in infrastructure, socioeconomic status, and the implementation of national STH control measures. The Parasitosis Prevention Law ended in 1994 and population-level screening ceased in Japan; therefore, current transmission status of STH in Japan is not well characterized. Sporadic cases of STH infections continue to be reported, raising the possibility of a larger-scale recrudescence of STH infections. Given that traditional microscopic detection methods are not sensitive to low-intensity STH infections, we conducted targeted prevalence surveys using sensitive PCR-based assays to evaluate the current STH-transmission status and to describe epidemiological characteristics of areas of Japan believed to have achieved historical elimination of STHs. Methods: Stool samples were collected from 682 preschool- and school-aged children from six localities of Japan with previously high prevalence of STH. Caregivers of participants completed a questionnaire to ascertain access to water, sanitation and hygiene (WASH), and potential exposures to environmental contamination. For fecal testing, multi-parallel real-time PCR assays were used to detect infections of Ascaris lumbricoides, Necator americanus, Ancylostoma duodenale and Trichuris trichiura. Results: Among the 682 children, no positive samples were identified, and participants reported high standards of WASH. Conclusions: To our knowledge, this is the first STH-surveillance study in Japan to use sensitive molecular techniques for STH detection. The results suggest that recrudescence of STH infections has not occurred, and that declines in prevalence have been sustained in the sampled areas. These findings suggest that reductions in prevalence below the elimination thresholds, suggestive of transmission interruption, are possible. Additionally, this study provides circumstantial evidence that multi-parallel real-time PCR methods are applicable for evaluating elimination status in areas where STH prevalence is extremely low.[Figure not available: see fulltext.]

Description:

We acknowledge the generous funding from the Natural History Museum, London, which is funded by the Bill and Melinda Gates Foundation (OPP1129535). Publisher Copyright: © 2020 The Author(s).

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