dc.contributor.author |
Helgadóttir, Helga |
dc.contributor.author |
Tropea, Teresa |
dc.contributor.author |
Gizurarson, Sveinbjörn |
dc.contributor.author |
Mandalà, Maurizio |
dc.date.accessioned |
2022-06-25T01:01:56Z |
dc.date.available |
2022-06-25T01:01:56Z |
dc.date.issued |
2021-09-21 |
dc.identifier.citation |
Helgadóttir , H , Tropea , T , Gizurarson , S & Mandalà , M 2021 , ' Endothelium-derived hyperpolarizing factor (Edhf) mediates acetylsalicylic acid (aspirin) vasodilation of pregnant rat mesenteric arteries ' , International Journal of Molecular Sciences , vol. 22 , no. 18 , 10162 . https://doi.org/10.3390/ijms221810162 |
dc.identifier.issn |
1661-6596 |
dc.identifier.other |
40221848 |
dc.identifier.other |
0db55ec2-994b-4ba8-a94d-98d448855996 |
dc.identifier.other |
85115191494 |
dc.identifier.other |
34576324 |
dc.identifier.other |
unpaywall: 10.3390/ijms221810162 |
dc.identifier.uri |
https://hdl.handle.net/20.500.11815/3263 |
dc.description |
Funding Information: Funding: This study was supported by the grants European Union 7th Framework Programme–FP7 (ASPRE Project # 601852); the Icelandic Research Fund (Rannís, no. 163369-051). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). |
dc.description.abstract |
Acetylsalicylic acid (aspirin) exhibits a broad range of activities, including analgesic, antipyretic, and antiplatelet properties. Recent clinical studies also recommend aspirin prophylaxis in women with a high risk of pre-eclampsia, a major complication of pregnancy characterized by hypertension. We investigated the effect of aspirin on mesenteric resistance arteries and found out-discovered the molecular mechanism underlying this action. Aspirin (10−12–10−6 M) was tested on pregnant rat mesenteric resistance arteries by a pressurized arteriography. Aspirin was investigated in the presence of several inhibitors of: (a) nitric oxide synthase (L-NAME 2 × 10−4 M); (b) cyclooxygenase (Indomethacin, 10−5 M); (c) Ca2+-activated K+ channels (Kca): small conductance (SKca, Apamin, 10−7 M), intermediate conductance (IKca, TRAM34, 10−5 M), and big conductance (BKca, paxilline, 10−5 M); and (d) endothelial-derived hyperpolarizing factor (high KCl, 80 mM). Aspirin caused a concentration-dependent vasodilation. Aspirin-vasodilation was abolished by removal of endothelium or by high KCl. Furthermore, preincubation with either apamin plus TRAM-34 or paxillin significantly attenuated aspirin vasodilation (p < 0.05). For the first time, we showed that aspirin induced endothelium-dependent vasodilation in mesenteric resistance arteries through the endothelial-derived hyperpolarizing factor (EDHF) and calcium-activated potassium channels. By activating this molecular mechanism, aspirin may lower peripheral vascular resistance and be beneficial in pregnancies complicated by hypertension. |
dc.format.extent |
1878807 |
dc.format.extent |
|
dc.language.iso |
en |
dc.relation |
info:eu-repo/grantAgreement/EC/FP7/601852 |
dc.relation.ispartofseries |
International Journal of Molecular Sciences; 22(18) |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.subject |
Animals |
dc.subject |
Aspirin/therapeutic use |
dc.subject |
Biological Factors/genetics |
dc.subject |
Mesenteric Arteries/drug effects |
dc.subject |
Myocytes, Smooth Muscle/drug effects |
dc.subject |
Rats |
dc.subject |
Rats, Sprague-Dawley |
dc.title |
Endothelium-derived hyperpolarizing factor (Edhf) mediates acetylsalicylic acid (aspirin) vasodilation of pregnant rat mesenteric arteries |
dc.type |
/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article |
dc.description.version |
Peer reviewed |
dc.identifier.doi |
10.3390/ijms221810162 |
dc.relation.url |
http://www.scopus.com/inward/record.url?scp=85115191494&partnerID=8YFLogxK |
dc.contributor.department |
Faculty of Pharmaceutical Sciences |