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Combining whole-cell patch clamp and dye loading in acute brain slices with bulk RNA sequencing in embryonic to aged mice

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dc.contributor.author Kamen, Yasmine
dc.contributor.author Káradóttir, Ragnhildur Þóra
dc.date.accessioned 2022-03-10T01:03:11Z
dc.date.available 2022-03-10T01:03:11Z
dc.date.issued 2021-06-18
dc.identifier.citation Kamen , Y & Káradóttir , R Þ 2021 , ' Combining whole-cell patch clamp and dye loading in acute brain slices with bulk RNA sequencing in embryonic to aged mice ' , STAR Protocols , vol. 2 , no. 2 , 100439 , pp. 100439 . https://doi.org/10.1016/j.xpro.2021.100439
dc.identifier.issn 2666-1667
dc.identifier.other PURE: 43318607
dc.identifier.other PURE UUID: 62dfc9cb-8487-478f-b101-54b1bc92bc39
dc.identifier.other Scopus: 85104149679
dc.identifier.uri https://hdl.handle.net/20.500.11815/2939
dc.description We would like to thank Dr. Maike Paramor for comments on the protocol, Kimberley Evans for comments on the protocol and assistance with the graphics, Dr Omar de Faria Jr for assistance with the figures, and our following funders: the European Research Council (ERC: the European Union’s Horizon 2020 research and innovation programme grant agreement No 771411; R.T.K.); the Wellcome (Studentship award 102160/Z/13/Z; Y.K.); the Fonds de recherche du Québec-Santé (a scholarship, Y.K.); The Cambridge Commonwealth European & International Trust (a scholarship, Y.K.); and the Lister Institute of Preventive Medicine (a research prize, R.T.K.). The funders had no role in decision to publish or preparation of the manuscript. Publisher Copyright: © 2021 The Author(s)
dc.description.abstract Single-cell electrophysiological recordings combined with dye loading and immunohistochemistry provide unparalleled single-cell resolution of cell physiology, morphology, location, and protein expression. When correlated with bulk RNA sequencing, these data can define cell identity and function. Here, we describe a protocol to prepare acute brain slices from embryonic and postnatal mice for whole-cell patch clamp, dye loading and post-hoc immunohistochemistry, and cell isolation for bulk RNA sequencing. While we focus on oligodendrocyte precursor cells, this protocol is applicable to other brain cells. For complete details on the use and execution of this protocol, please refer to Spitzer et al. (2019).
dc.format.extent 100439
dc.language.iso en
dc.relation info:eu-repo/grantAgreement/EC/H2020/771411
dc.relation.ispartofseries STAR Protocols; 2(2)
dc.rights info:eu-repo/semantics/openAccess
dc.subject Taugalækningar
dc.subject Sameindalíffræði
dc.subject Raðgreining
dc.subject Stofnfrumur
dc.subject Cell isolation
dc.subject Microscopy
dc.subject Molecular Biology
dc.subject Neuroscience
dc.subject RNA-seq
dc.subject Stem Cells
dc.subject Biochemistry, Genetics and Molecular Biology (all)
dc.subject Neuroscience (all)
dc.subject Immunology and Microbiology (all)
dc.title Combining whole-cell patch clamp and dye loading in acute brain slices with bulk RNA sequencing in embryonic to aged mice
dc.type /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article
dc.description.version Peer reviewed
dc.identifier.pmid 33899020
dc.identifier.doi https://doi.org/10.1016/j.xpro.2021.100439
dc.relation.url http://www.scopus.com/inward/record.url?scp=85104149679&partnerID=8YFLogxK
dc.contributor.department Faculty of Medicine


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