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The effect of sample age on the metabolic information extracted from formalin-fixed and paraffin embedded tissue samples using desorption electrospray ionization mass spectrometry imaging

The effect of sample age on the metabolic information extracted from formalin-fixed and paraffin embedded tissue samples using desorption electrospray ionization mass spectrometry imaging


Title: The effect of sample age on the metabolic information extracted from formalin-fixed and paraffin embedded tissue samples using desorption electrospray ionization mass spectrometry imaging
Author: Ísberg, Ólöf Gerður
Xiang, Yuchen
Böðvarsdóttir, Sigríður Klara
Jónasson, Jón Gunnlaugur
Þorsteinsdóttir, Margrét
Takats, Zoltan
Date: 2021-11
Language: English
Scope: 6
University/Institute: Landspitali - The National University Hospital of Iceland
Department: Faculty of Medicine
Clinical Laboratory Services, Diagnostics and Blood Bank
Faculty of Pharmaceutical Sciences
Series: Journal of Mass Spectrometry and Advances in the Clinical Lab; 22()
ISSN: 2667-145X
DOI: https://doi.org/10.1016/j.jmsacl.2021.10.004
Subject: Litrófsgreining; Massagreining; Massagreining; Lífefnafræði; Meinafræði; DESI-MSI; FFPE; Lipids; Mass spectrometry imaging; Sample age; Spectroscopy; Medical Laboratory Technology; Clinical Biochemistry; Microbiology
URI: https://hdl.handle.net/20.500.11815/2929

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Citation:

Ísberg , Ó G , Xiang , Y , Böðvarsdóttir , S K , Jónasson , J G , Þorsteinsdóttir , M & Takats , Z 2021 , ' The effect of sample age on the metabolic information extracted from formalin-fixed and paraffin embedded tissue samples using desorption electrospray ionization mass spectrometry imaging ' , Journal of Mass Spectrometry and Advances in the Clinical Lab , vol. 22 , pp. 50-55 . https://doi.org/10.1016/j.jmsacl.2021.10.004

Abstract:

Background: Metabolites, especially lipids, have been shown to be promising therapeutic targets. In conjugation with genes and proteins they can be used to identify phenotypes of disease and support the development of targeted treatments. The majority of clinically collected tissue samples are stored in formalin-fixed and paraffin embedded (FFPE) blocks due to their tissue conservation ability and indefinite storage capacity. For metabolic analysis, however, fresh frozen (FF) samples are currently preferred over FFPE samples due to concerns of metabolic information being lost when preparing the samples. With little or no sample preparation, desorption electrospray ionisation mass spectrometry imaging (DESI-MSI) allows for the study of spatial as well as spectral information. Methods: DESI-MSI analysis was performed on FFPE breast cancer tissue microarray samples from 213 patients collected between the years 1935-2013. Logistic regression (LR) models were built to classify samples based on age and FF samples were used for feature validation. Results: LR models developed on the FFPE samples achieved an average classification accuracy of 96% when predicting their age with a 10-year grouping. Closer examination of the metabolic change over time revealed that the mean signal intensities for the lower mass range (100 - 500 m/z) linearly decrease over time, while the mean intensities for the higher mass range (500 - 900 m/z), remained relatively constant. Conclusions: In our samples, which span over 70 years, sample age has a weak yet quantifiable impact on metabolite content in FFPE samples, while the higher mass range is seemingly unaffected. FFPE samples thus provide an alternative avenue for metabolic analysis of lipids.

Description:

Funding Information: This document is the result of the research project funded by the Icelandic Centre for Research, grant no. 174566051 and by the Icelandic Breast Cancer Research Fund, Göngum Saman. Publisher Copyright: © 2021 THE AUTHORS

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