Title: | Xylanolytic psychotrophs from andosolic sedge fens and moss heaths in Iceland |
Author: |
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Date: | 2018-12-21 |
Language: | English |
Scope: | 171-186 |
University/Institute: | Háskólinn á Akureyri University of Akureyri |
School: | Viðskipta- og raunvísindasvið (HA) School of Business and Science (UA) |
Department: | Auðlindadeild (HA) Faculty of Natural Resource Sciences (UA) |
Series: | Fine Focus;4(2) |
ISSN: | 2381-0637 |
DOI: | 10.33043/FF.4.2.171-186 |
Subject: | Gerlar; Mýrlendi; Mosar; Jarðvegsrannsóknir; Bacteria; Fen; Moss; Soil investigation |
URI: | https://hdl.handle.net/20.500.11815/2420 |
Citation:Rickman, O. J., Sigurbjörnsdóttir, M. A. og Vilhemsson, O. (2018). Xylanolytic psychotrophs from andosolic sedge fens and moss heaths in Iceland. Fine Focus, 4(2), 171-186. https://doi.org/10.33043/FF.4.2.171-186
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Abstract:Nine xylanolytic bacterial strains were isolated from fen and heath soils in northern Iceland. They were found by 16S rRNA gene sequencing to belong to the genera Paenibacillus, Bacillus, Pseudomonas, and Stenotrophomonas. Using a simple, plate-based semiquantitative assay with azo-crosslinked xylan as the substrate, it was determined that although isolated from cold environments, most of the strains displayed greater xylanolytic activity under mesophilic conditions, with only the paenibacilli displaying markedly cold-active xylanolytic activity. Indeed, for one isolate, Paenibacillus castaneae OV2122, xylanolytic activity was only detected at 15°C and below under the conditions tested. Of the nine strains, Paenibacillus amylolyticus OV2121 displayed the greatest activity at 5°C. Glycohydrolase family-specific PCR indicated that the paenibacilli produced multiple xylanases of families 10 and 11, whereas a family 8 xylanase was detected in Pseudomonas kilonensis AL1515, and a family 11 xylanase in Stenotrophomonas rhizophila AL1610.
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Rights:By submitting to Fine Focus, the author(s) agree to the terms of the Author Agreement. Beginning in Fall 2018, all authors retain copyrights associated with their article contributions and agree to make such contributions available under a Creative Commons Attribution-NonCommercial 4.0 International license upon publication in Fine Focus. Copyrights to articles published prior to Fall 2018 have been transferred from the authors to Fine Focus.
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