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Xylanolytic psychotrophs from andosolic sedge fens and moss heaths in Iceland

Xylanolytic psychotrophs from andosolic sedge fens and moss heaths in Iceland


Title: Xylanolytic psychotrophs from andosolic sedge fens and moss heaths in Iceland
Author: Rickman, Olivia   orcid.org/0000-0002-7842-1150
Sigurbjörnsdóttir, M. Auður   orcid.org/0000-0001-5725-2020
Vilhelmsson, Oddur   orcid.org/0000-0001-8799-0964
Date: 2018-12-21
Language: English
Scope: 171-186
University/Institute: Háskólinn á Akureyri
University of Akureyri
School: Viðskipta- og raunvísindasvið (HA)
School of Business and Science (UA)
Department: Auðlindadeild (HA)
Faculty of Natural Resource Sciences (UA)
Series: Fine Focus;4(2)
ISSN: 2381-0637
DOI: 10.33043/FF.4.2.171-186
Subject: Gerlar; Mýrlendi; Mosar; Jarðvegsrannsóknir; Bacteria; Fen; Moss; Soil investigation
URI: https://hdl.handle.net/20.500.11815/2420

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Citation:

Rickman, O. J., Sigurbjörnsdóttir, M. A. og Vilhemsson, O. (2018). Xylanolytic psychotrophs from andosolic sedge fens and moss heaths in Iceland. Fine Focus, 4(2), 171-186. https://doi.org/10.33043/FF.4.2.171-186

Abstract:

Nine xylanolytic bacterial strains were isolated from fen and heath soils in northern Iceland. They were found by 16S rRNA gene sequencing to belong to the genera Paenibacillus, Bacillus, Pseudomonas, and Stenotrophomonas. Using a simple, plate-based semiquantitative assay with azo-crosslinked xylan as the substrate, it was determined that although isolated from cold environments, most of the strains displayed greater xylanolytic activity under mesophilic conditions, with only the paenibacilli displaying markedly cold-active xylanolytic activity. Indeed, for one isolate, Paenibacillus castaneae OV2122, xylanolytic activity was only detected at 15°C and below under the conditions tested. Of the nine strains, Paenibacillus amylolyticus OV2121 displayed the greatest activity at 5°C. Glycohydrolase family-specific PCR indicated that the paenibacilli produced multiple xylanases of families 10 and 11, whereas a family 8 xylanase was detected in Pseudomonas kilonensis AL1515, and a family 11 xylanase in Stenotrophomonas rhizophila AL1610.

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