Háskóli ÍslandsUniversity of IcelandGuinde, JulienBenoit, AudreyFrankel, DianeRobert, StéphaneOstacolo, KevinLévy, NicolasAstoul, PhilippeRoll, PatriceKaspi, Elise2021-01-282021-01-282020-02-26Guinde J, Benoit A, Frankel D, Robert S, Ostacolo K, Lévy N, Astoul P, Roll P, Kaspi E. miR-9 Does Not Regulate Lamin A Expression in Metastatic Cells from Lung Adenocarcinoma. International Journal of Molecular Sciences. 2020; 21(5):1599. https://doi.org/10.3390/ijms210515991422-0067https://hdl.handle.net/20.500.11815/2429Publisher's version (útgefin grein)In lung adenocarcinoma, low lamin A expression in pleural metastatic cells has been proposed as a pejorative factor. miR-9 physiologically inhibits the expression of lamin A in neural cells and seems to be a central actor in the carcinogenesis and the metastatic process in lung cancer. Thus, it could be a good candidate to explain the reduction of lamin A expression in lung adenocarcinoma cells. miR-9 expression was analyzed in 16 pleural effusions containing metastatic cells from lung adenocarcinoma and was significantly reduced in patients from the ‘Low lamin A expression’ group compared to patients from the ‘High lamin A expression’ group. Then, carcinoma cells selection by fluorescence-activated cell sorting (FACS) was performed according to epithelial membrane antigen (EMA) expression, reflecting lamin A expression. miR-9 was underexpressed in lamin A– carcinoma cells compared to lamin A+ carcinoma cells in patients from the ‘Low lamin A expression’ group, whereas there was no difference of miR-9 expression between lamin A+ and lamin A– carcinoma cells in patients from the ‘High lamin A expression’ group. These results suggest that miR-9 does not regulate lamin A expression in metastatic cells from lung adenocarcinoma. On the contrary, miR-9 expression was shown to be reduced in lamin A-negative carcinoma cells.1599eninfo:eu-repo/semantics/openAccessLamin ALung adenocarcinomaMicroRNA-9Pleural effusionsLungnasjúkdómarinfo:eu-repo/semantics/articleInternational Journal of Molecular Sciences10.3390/ijms21051599