Orlygsson, JohannScully, Sean Michael2025-11-142025-11-142024-02Orlygsson, J & Scully, S M 2024, 'Dataset describing the amino acid catabolism of Thermoanaerobacter pseudethanolicus', Data in Brief, vol. 52, 110017. https://doi.org/10.1016/j.dib.2023.1100172352-34092455412722d410200-5b15-4b2c-9056-e75a486e00fd85181671999https://hdl.handle.net/20.500.11815/5932©2023 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license ( http://creativecommons.org/licenses/by/4.0/ )The dataset depicts the catabolism of branched-chain amino acids by Thermoanaerobacter pseudethanolicus in the presence of thiosulfate under different culture conditions. The results reveal that the strain can degrade all three branched-chain amino acids resulting in the production of their corresponding branched-chain fatty acids and branched-chain alcohols with the fatty acids always being the dominant product. The highest amounts of 2-methyl-1-butanol from isoleucine were at pH 6.5, liquid-gas ratio of 0.98, and at 20 mM thiosulfate concentration. A kinetic experiment of the branched-chain amino acids was done in the presence of thiosulfate as are data on selected enzyme activities related to alcohols and aldehydes. Finally, an NMR study using 13C1 methyl-1-butyrate during the degradation of leucine in the presence of thiosulfate was done to prove that the 13C1-methyl-1-butanol was indeed from its corresponding fatty acid.956440eninfo:eu-repo/semantics/openAccessAlcohols, and alcoholsAnaerobicBranched-chain amino acidsThermophilicThiosulfateMultidisciplinary/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article10.1016/j.dib.2023.110017