Háskóli ÍslandsUniversity of IcelandShelke, SandipSandholt, Gunnar BirgirSigurdsson, Snorri2020-08-102020-08-102014S. A. Shelke; G. B. Sandholt; S. Sigurdsson, „Nitroxide-labeled pyrimidines for non-covalent spinlabeling of abasic sites in DNA and RNA duplexes,“ in Organic & Biomolecular Chemistry, vol. 12, no.37, pp. 7366-7374 10.1039/c4ob01095g1477-05201477-0539 (eISSN)https://hdl.handle.net/20.500.11815/1952Post-print (lokagerð höfundar)Non-covalent and site-directed spin labeling gives easy access to spin-labeled nucleic acids for the study of their structure and dynamics by electron paramagnetic resonance (EPR) spectroscopy. In a search for improved spin labels for non-covalent binding to abasic sites in duplex DNA and RNA, ten pyrimidine-derived spin labels were prepared in good yields and their binding was evaluated by continuous wave (CW)-EPR spectroscopy. Most of the spin labels showed lower binding affinity than the previously reported label c towards abasic sites in DNA and RNA. The most promising labels were triazole-linked spin labels and a pyrrolocytosine label. In particular, the N1-ethylamino derivative of a triazole-linked uracil spin label binds fully to both DNA and RNA containing an abasic site. This is the first example of a spin label that binds fully through non-covalent interactions with an abasic site in RNA.7366-7374eninfo:eu-repo/semantics/openAccessCleave apurinic sitesDistance measurementNucleic acidEPR SpectroscopyClick chemistryFluorescenceNucleosideLigandKjarnsýrurLitrófsgreiningSameindaerfðafræðiinfo:eu-repo/semantics/articleOrganic and Biomolecular Chemistry10.1039/C4OB01095G