Site-directed spin labeling of 2′-amino groups in RNA with isoindoline nitroxides that are resistant to reduction

Háskóli ÍslandsUniversity of IcelandSaha, SubhamJagtap, Anil P.Sigurdsson, Snorri2020-08-102020-08-102015Saha, S., Jagtap, A. P., & Sigurdsson, S. T. (2015). Site-directed spin labeling of 2′-amino groups in RNA with isoindoline nitroxides that are resistant to reduction. Chemical Communications, 51(66), 13142-13145 doi:10.1039/c5cc05014f1359-73451364-548X (eISSN)https://hdl.handle.net/20.500.11815/1957Post-print (lokagerð höfundar)Two aromatic isothiocyanates, derived from isoindoline nitroxides, were synthesized and selectively reacted with 2′-amino groups in RNA. The spin labels displayed limited mobility in RNA, making them promising candidates for distance measurements by pulsed EPR. After conjugation to RNA, a tetraethyl isoindoline derivative showed significant stability under reducing conditions.13142-13145eninfo:eu-repo/semantics/openAccessNucleic acid structureIn-cell EPRDuplex dnaDynamics perspectivesDistance measurementsAbasic sitesProbeSpectroscopyNoncovalentKjarnsýrurLitrófsgreiningDNA-rannsóknirSite-directed spin labeling of 2′-amino groups in RNA with isoindoline nitroxides that are resistant to reductioninfo:eu-repo/semantics/articleChemical Communications10.1039/C5CC05014F