Site-directed spin labeling of 2′-amino groups in RNA with isoindoline nitroxides that are resistant to reduction

Saha, Subham; Jagtap, Anil P.; Sigurdsson, Snorri

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dc.contributor	Háskóli Íslands
dc.contributor	University of Iceland
dc.contributor.author	Saha, Subham
dc.contributor.author	Jagtap, Anil P.
dc.contributor.author	orcid.org/0000-0003-2492-1456 Sigurdsson, Snorri
dc.date.accessioned	2020-08-10T15:15:19Z
dc.date.available	2020-08-10T15:15:19Z
dc.date.issued	2015
dc.identifier.citation	Saha, S., Jagtap, A. P., & Sigurdsson, S. T. (2015). Site-directed spin labeling of 2′-amino groups in RNA with isoindoline nitroxides that are resistant to reduction. Chemical Communications, 51(66), 13142-13145 doi:10.1039/c5cc05014f
dc.identifier.issn	1359-7345
dc.identifier.issn	1364-548X (eISSN)
dc.identifier.uri	https://hdl.handle.net/20.500.11815/1957
dc.description	Post-print (lokagerð höfundar)
dc.description.abstract	Two aromatic isothiocyanates, derived from isoindoline nitroxides, were synthesized and selectively reacted with 2′-amino groups in RNA. The spin labels displayed limited mobility in RNA, making them promising candidates for distance measurements by pulsed EPR. After conjugation to RNA, a tetraethyl isoindoline derivative showed significant stability under reducing conditions.
dc.description.sponsorship	This work was supported by the Icelandic Research Fund (141062-051). S.S. and A.P.J. gratefully acknowledge doctoral fellowships provided by the University of Iceland. The authors thank Dr S. Jonsdottir for assistance with collecting analytical data for structural characterization of compounds prepared and members of the Sigurdsson research group for critical reading of the manuscript.
dc.format.extent	13142-13145
dc.language.iso	en
dc.publisher	Royal Society of Chemistry (RSC)
dc.relation.ispartofseries	Chemical Communications;51(66)
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	Nucleic acid structure
dc.subject	In-cell EPR
dc.subject	Duplex dna
dc.subject	Dynamics perspectives
dc.subject	Distance measurements
dc.subject	Abasic sites
dc.subject	Probe
dc.subject	Spectroscopy
dc.subject	Noncovalent
dc.subject	Kjarnsýrur
dc.subject	Litrófsgreining
dc.subject	DNA-rannsóknir
dc.title	Site-directed spin labeling of 2′-amino groups in RNA with isoindoline nitroxides that are resistant to reduction
dc.type	info:eu-repo/semantics/article
dc.description.version	Peer reviewed
dc.identifier.journal	Chemical Communications
dc.identifier.doi	10.1039/C5CC05014F
dc.relation.url	http://pubs.rsc.org/en/content/articlepdf/2015/CC/C5CC05014F
dc.contributor.department	Raunvísindadeild (HÍ)
dc.contributor.department	Faculty of Physical Sciences (UI)
dc.contributor.department	Raunvísindastofnun (HÍ)
dc.contributor.department	Science Institute (UI)
dc.contributor.school	Verkfræði- og náttúruvísindasvið (HÍ)
dc.contributor.school	School of Engineering and Natural Sciences (UI)