dc.contributor |
Háskóli Íslands |
dc.contributor |
University of Iceland |
dc.contributor.author |
Kamble, Nilesh Ramesh |
dc.contributor.author |
Wolfrum, Manpreet |
dc.contributor.author |
Halbritter, Thomas |
dc.contributor.author |
Sigurdsson, Snorri |
dc.contributor.author |
Richert, Clemens |
dc.date.accessioned |
2020-08-07T14:06:50Z |
dc.date.available |
2020-08-07T14:06:50Z |
dc.date.issued |
2020-01-21 |
dc.identifier.citation |
Kamble, N., Wolfrum, M., Halbritter, T., Sigurdsson, S. T., & Richert, C. (2020). Noncovalent Spin-Labeling of DNA and RNA Triplexes. 17(2), e1900676. doi:10.1002/cbdv.201900676 |
dc.identifier.issn |
1612-1872 |
dc.identifier.issn |
1612-1880 (eISSN) |
dc.identifier.uri |
https://hdl.handle.net/20.500.11815/1938 |
dc.description |
Publisher's version (útgefin grein) |
dc.description.abstract |
Studying nucleic acids often requires labeling. Many labeling approaches require covalent bonds between the nucleic acid and the label, which complicates experimental procedures. Noncovalent labeling avoids the need for highly specific reagents and reaction conditions, and the effort of purifying bioconjugates. Among the least invasive techniques for studying biomacromolecules are NMR and EPR. Here, we report noncovalent labeling of DNA and RNA triplexes with spin labels that are nucleobase derivatives. Spectroscopic signals indicating strong binding were detected in EPR experiments in the cold, and filtration assays showed micromolar dissociation constants for complexes between a guanine‐derived label and triplex motifs containing a single‐nucleotide gap in the oligopurine strand. The advantages and challenges of noncovalent labeling via this approach that complements techniques relying on covalent links are discussed. |
dc.description.sponsorship |
The authors thank Anna‐Lena J. Segler for technical assistance. This work was supported by the Icelandic Research Fund, grant No. 141062‐051 to S.T.S. and DFG, grant No. RI 1063/16‐1 to C.R. T.H. thanks the Deutsche Forschungsgemeinschaft (DFG) for a postdoctoral fellowship (414196920). |
dc.format.extent |
e1900676 |
dc.language.iso |
en |
dc.publisher |
Wiley |
dc.relation.ispartofseries |
Chemistry & Biodiversity;17(2) |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.subject |
DNA |
dc.subject |
RNA |
dc.subject |
Triplexes |
dc.subject |
Spin labeling |
dc.subject |
EPR |
dc.subject |
Kjarnsýrur |
dc.title |
Noncovalent Spin‐Labeling of DNA and RNA Triplexes |
dc.type |
info:eu-repo/semantics/article |
dcterms.license |
This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
dc.description.version |
Peer reviewed |
dc.identifier.journal |
Chemistry & Biodiversity |
dc.identifier.doi |
10.1002/cbdv.201900676 |
dc.contributor.department |
Raunvísindadeild (HÍ) |
dc.contributor.department |
Faculty of Physical Sciences (UI) |
dc.contributor.department |
Raunvísindastofnun (HÍ) |
dc.contributor.department |
Science Institute (UI) |
dc.contributor.school |
Verkfræði- og náttúruvísindasvið (HÍ) |
dc.contributor.school |
School of Engineering and Natural Sciences (UI) |