dc.contributor |
Háskóli Íslands |
dc.contributor |
University of Iceland |
dc.contributor.author |
Guðmundsson, Bjarki |
dc.contributor.author |
Þormar, Hans G |
dc.contributor.author |
Sigurðsson, Albert |
dc.contributor.author |
Dankers, Wendy |
dc.contributor.author |
Steinarsdóttir, Margrét |
dc.contributor.author |
Hermanowicz, Stefan |
dc.contributor.author |
Sigurðsson, Stefán |
dc.contributor.author |
Ólafsson, Davið |
dc.contributor.author |
Halldorsdottir, Anna |
dc.contributor.author |
Meyn, Stephen |
dc.contributor.author |
Jonsson, Jon J. |
dc.date.accessioned |
2019-12-05T15:18:24Z |
dc.date.available |
2019-12-05T15:18:24Z |
dc.date.issued |
2018-07-24 |
dc.identifier.citation |
Bjarki Gudmundsson, Hans G Thormar, Albert Sigurdsson, Wendy Dankers, Margret Steinarsdottir, Stefan Hermanowicz, Stefan Sigurdsson, David Olafsson, Anna M Halldorsdottir, Stephen Meyn, Jon J Jonsson, Northern lights assay: a versatile method for comprehensive detection of DNA damage, Nucleic Acids Research, Volume 46, Issue 20, 16 November 2018, Page e118, https://doi.org/10.1093/nar/gky645 |
dc.identifier.issn |
0305-1048 |
dc.identifier.issn |
1362-4962 (eISSN) |
dc.identifier.uri |
https://hdl.handle.net/20.500.11815/1373 |
dc.description |
Publisher's version (útgefin grein) |
dc.description.abstract |
DNA damage assays have various limitations in types of lesions detected, sensitivity, specificity and samples that can be analyzed. The Northern Lights Assay (NLA) is based on 2D Strandness-Dependent Electrophoresis (2D-SDE), a technique that separates nucleic acids based on length, strandness, structure and conformation changes induced by damage. NLA is run on a microgel platform in 20- 25 min. Each specimen is analyzed in pairs of nondigested DNA to detect single- and double-stranded breaks (DSBs) and Mbo I-digested DNA to detect other lesions. We used NLA to evaluate DNA in solution and isolated from human cells treated with various genotoxic agents. NLA detected and distinguished between single- and DSBs, interstrand and intrastrand DNA crosslinks, and denatured singlestranded DNA. NLA was sufficiently sensitive to detect biologically relevant amount of DNA damage. NLA is a versatile, sensitive and simple method for comprehensive and simultaneous analysis of multiple types of damage, both in purified DNA and in DNA isolated from cells and body fluids. NLA can be used to evaluate DNA quality in biosamples, monitor complex molecular procedures, assess genotoxicity, diagnose genome instability, facilitate cancer theranostics and in basic nucleic acids research. |
dc.description.sponsorship |
We wish to thank Agata Smogorzewska and Supawat
Thongthip (The Laboratory of Genome Maintenance,
Rockefeller University, New York, USA), for the kind gift
of FA cell lines and control cell lines.
University of Iceland Research Fund [210757-5389]; Landspitali University Hospital Research Fund; Icelandic Center for Research Funds [1427090611]; L´ıfeind ehf. (to B.G)
(in part), (to H.G.Th.). Funding for open access charge:
University of Iceland Research Fund.
Conflict of interest statement. B.G., H.G.Th. and J.J.J. are
stockholders in Lifeind ehf., a company that holds the
patent to 2D-SDE and is marketing the NLA. J.J.J. receives
research funding from Lifeind ehf. and H.G.Th. is employed
by Lifeind ehf. |
dc.format.extent |
e118 |
dc.language.iso |
en |
dc.publisher |
Oxford University Press (OUP) |
dc.relation.ispartofseries |
Nucleic Acids Research;46(20) |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.subject |
Genetics |
dc.subject |
Cell DNA |
dc.subject |
Genomic DNA |
dc.subject |
Nucleic acid |
dc.subject |
Erfðafræði |
dc.subject |
DNA-rannsóknir |
dc.title |
Northern lights assay: a versatile method for comprehensive detection of DNA damage |
dc.type |
info:eu-repo/semantics/article |
dcterms.license |
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
dc.description.version |
Peer reviewed |
dc.identifier.journal |
Nucleic Acids Research |
dc.identifier.doi |
10.1093/nar/gky645 |
dc.relation.url |
http://academic.oup.com/nar/advance-article-pdf/doi/10.1093/nar/gky645/25204881/gky645.pdf |
dc.contributor.department |
Faculty of Medicine (UI) |
dc.contributor.department |
The Biomedical Center (BMC) (UI) |
dc.contributor.department |
Lífvísindasetur (HÍ) |
dc.contributor.department |
Læknadeild (HÍ) |
dc.contributor.school |
Heilbrigðisvísindasvið (HÍ) |
dc.contributor.school |
School of Health Sciences (UI) |