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Transplanting embryonic stem cells onto damaged human corneal endothelium

Transplanting embryonic stem cells onto damaged human corneal endothelium


Title: Transplanting embryonic stem cells onto damaged human corneal endothelium
Author: Hanson, Charles
Arnarsson, Arsaell   orcid.org/0000-0002-5804-8416
Hardarson, Thorir
Lindgård, Ann
Daneshvarnaeini, Mandana
Ellerström, Catarina
Bruun, Anita
Stenevi, Ulf
Date: 2017-08-26
Language: English
Scope: 127
University/Institute: Háskólinn á Akureyri
University of Iceland
School: Hug- og félagsvísindasvið (HA)
School of Humanities and Social Sciences (UA)
Department: Félagsvísindadeild (HA)
Faculty of Social Sciences (UA)
Series: World Journal of Stem Cells;9(8)
ISSN: 1948-0210 (eISSN)
DOI: 10.4252/wjsc.v9.i8.127
Subject: Stem cells; Cornea; Stofnfrumur; Hornhimna
URI: https://hdl.handle.net/20.500.11815/1370

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Citation:

Hanson, C., Arnarsson, A., Hardarson, T., Lindgård, A., Daneshvarnaeini, M., Ellerström, C., . . . Stenevi, U. (2017). Transplanting embryonic stem cells onto damaged human corneal endothelium. World Journal of Stem Cells, 9(8), 127-132. doi:10.4252/wjsc.v9.i8.127

Abstract:

AIM: To investigate whether human embryonic stem cells (hESCs) could be made to attach, grow and differentiate on a human Descemet's membrane (DM). Spontaneously differentiated hESCs were transferred onto a human corneal button with the endothelial layer removed using ocular sticks. The cells were cultured on a DM for up to 15 d. The genetically engineered hESC line expressed green fluorescent protein, which facilitated identification during the culture experiments, tissue preparation, and analysis. To detect any differentiation into human corneal endothelial-like cells, we analysed the transplanted cells by immunohistochemistry using specific antibodies. RESULTS: We found transplanted cells form a single layer of cells with a hexagonal shape in the periphery of the DM. The majority of the cells were negative for octamer-binding transcription factor 4 but positive for paired box 6 protein, sodium potassium adenosine triphosphatase (NaKATPase), and Zona Occludens protein 1. In four of the 18 trials, the transplanted cells were found to express CK3, which indicates that the stem cells differentiated into corneal epithelial cells in these cases. CONCLUSION: It is possible to get cells originating from hESCs to become established on a human DM, where they grow and differentiate into corneal endothelial-like cells in vitro.

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This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/ licenses/by-nc/4.0/

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