Opin vísindi
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Distance measurements between manganese(ii) and nitroxide spin-labels by DEER determine a binding site of Mn2+ in the HP92 loop of ribosomal RNA

Kaminker, Ilia; Bye, Morgan; Mendelman, Natanel; Gislason, Kristmann; Sigurdsson, Snorri; Goldfarb, Daniella (Royal Society of Chemistry (RSC), 2015)

Mn2+ localization in hairpin 92 of the 23S ribosomal RNA (HP92) was obtained using W-band (95 GHz) DEER (double electron-electron resonance) distance measurements between the Mn2+ ion and nitroxide spin labels on the RNA. It was found to be preferably ...
Site-directed spin labeling of 2′-amino groups in RNA with isoindoline nitroxides that are resistant to reduction

Saha, Subham; Jagtap, Anil P.; Sigurdsson, Snorri (Royal Society of Chemistry (RSC), 2015)

Two aromatic isothiocyanates, derived from isoindoline nitroxides, were synthesized and selectively reacted with 2′-amino groups in RNA. The spin labels displayed limited mobility in RNA, making them promising candidates for distance measurements by ...

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