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Browsing by Subject "Distance measurements"

Browsing by Subject "Distance measurements"

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    Determination of helix orientations in a flexible DNA by multi-frequency EPR spectroscopy 
    Grytz, C. M.; Kazemi, S.; Marko, A.; Cekan, P.; Güntert, P.; Sigurdsson, Snorri; Prisner, Thomas F. (Royal Society of Chemistry (RSC), 2017)
    Distance measurements are performed between a pair of spin labels attached to nucleic acids using Pulsed Electron–Electron Double Resonance (PELDOR, also called DEER) spectroscopy which is a complementary tool to other structure determination methods ...
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    Noncovalent and site-directed spin labeling of duplex RNA 
    Kamble, Nilesh Ramesh; Gränz, Markus; Prisner, Thomas F.; Sigurdsson, Snorri (Royal Society of Chemistry (RSC), 2016)
    An isoindoline-nitroxide derivative of guanine (G, "G-spin") was shown to bind specifically and effectively to abasic sites in duplex RNAs. Distance measurements on a G-labeled duplex RNA with PELDOR (DEER) showed a strong orientation dependence. Thus, ...
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    Noncovalent spin-labeling of RNA: the aptamer approach 
    Saha, Subham; Hetzke, Thilo; Prisner, Thomas F.; Sigurdsson, Snorri (Royal Society of Chemistry (RSC), 2018)
    In the first example of site-directed spin-labeling of unmodified RNA, a pyrrolidine-nitroxide derivative of tetramethylrosamine (TMR) was shown to bind with high affinity to the malachite green (MG) aptamer, as determined by continuous-wave (CW) ...
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    Pulsed EPR dipolar spectroscopy at Q- and G-band on a trityl biradical 
    Akhmetzyanov, D.; Schöps, P.; Marko, A.; Kunjir, N. C.; Sigurdsson, Snorri; Prisner, Thomas F. (Royal Society of Chemistry (RSC), 2015)
    Pulsed electron paramagnetic resonance (EPR) spectroscopy is a valuable technique for the precise determination of distances between paramagnetic spin labels that are covalently attached to macromolecules. Nitroxides have commonly been utilised as ...
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    Site-directed spin labeling of 2′-amino groups in RNA with isoindoline nitroxides that are resistant to reduction 
    Saha, Subham; Jagtap, Anil P.; Sigurdsson, Snorri (Royal Society of Chemistry (RSC), 2015)
    Two aromatic isothiocyanates, derived from isoindoline nitroxides, were synthesized and selectively reacted with 2′-amino groups in RNA. The spin labels displayed limited mobility in RNA, making them promising candidates for distance measurements by ...